Table 1 Escherichia coli strains and plasmids used in this study
Strains or plasmids | Genotypes and comments | Source or reference |
|---|---|---|
Strain | ||
DH5α | Routine cloning host | In-house collection |
BW25113 | rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1 | CGSC* |
BW25113ΔnadC | BW25113 with chromosomal nadC deletion | This study |
BW25113ΔnadC ΔpncA | BW25113 with chromosomal nadC and pncA deletion | This study |
BW25113ΔnadC ΔpncA ΔxapA | BW25113 with chromosomal nadC, pncA, and xapA deletion | This study |
BW25113ΔnadC ΔpncA ΔnadR | BW25113 with chromosomal nadC, pncA, and nadR deletion | This study |
BW25113ΔnadC ΔpncA ΔxapA ΔnadR | BW25113 with chromosomal nadC, pncA, xapA and nadR deletion | This study |
Plasmid | ||
pKD13 | Gene knockout procedure | CGSC* |
pKD46 | Gene knockout procedure | CGSC* |
pCP20 | Gene knockout procedure | CGSC* |
pBAD-hisA | bla + | In-house collection |
pBAD-EGFP | pBAD-hisA with EGFP gene | This study |
pBAD-xapA | pBAD-hisA with xapA gene | This study |
pET28a | Kana + | In-house collection |
pET28-xapA | pET28a with xapA gene | This study |
pEGFP-N2 | Template for PCR amplification of EGFP gene | In-house collection |