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Table 1 Escherichia coli strains and plasmids used in this study

From: New function for Escherichia coli xanthosine phophorylase (xapA): genetic and biochemical evidences on its participation in NAD+ salvage from nicotinamide

Strains or plasmids Genotypes and comments Source or reference
Strain
DH5α Routine cloning host In-house collection
BW25113 rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1 CGSC*
BW25113ΔnadC BW25113 with chromosomal nadC deletion This study
BW25113ΔnadC ΔpncA BW25113 with chromosomal nadC and pncA deletion This study
BW25113ΔnadC ΔpncA ΔxapA BW25113 with chromosomal nadC, pncA, and xapA deletion This study
BW25113ΔnadC ΔpncA ΔnadR BW25113 with chromosomal nadC, pncA, and nadR deletion This study
BW25113ΔnadC ΔpncA ΔxapA ΔnadR BW25113 with chromosomal nadC, pncA, xapA and nadR deletion This study
Plasmid
pKD13 Gene knockout procedure CGSC*
pKD46 Gene knockout procedure CGSC*
pCP20 Gene knockout procedure CGSC*
pBAD-hisA bla + In-house collection
pBAD-EGFP pBAD-hisA with EGFP gene This study
pBAD-xapA pBAD-hisA with xapA gene This study
pET28a Kana + In-house collection
pET28-xapA pET28a with xapA gene This study
pEGFP-N2 Template for PCR amplification of EGFP gene In-house collection
  1. *CGSC is the E. coli Genetic Stock Center of Yale University.