Functional characterization of probiotic surface layer protein-carrying Lactobacillus amylovorus strains

BMC Microbiology

Table 1 Strains used in this study

Strain	Reference or source	S-layer protein
Strain	Reference or source	Name	Calculated Mw^a (kDa)
Lactobacillus amylovorus DSM 16698	porcine faeces [24]	SlpA	44
		SlpB	50
		SlpC	40
Lactobacillus amylovorus DSM 20531^T	DSM, fermented corn silage	SlpA	44
Lactobacillus amylovorus GRL 1112 (LAB 2)	porcine faeces [28]	SlpA	45
Lactobacillus amylovorus GRL 1114 (LAB 8)	porcine faeces [28]	SlpA	43
Lactobacillus amylovorus GRL 1115 (LAB 13)	porcine ileum [28]	SlpA	47
Lactobacillus amylovorus GRL 1116 (LAB 16)	porcine jejunum [28]	SlpA	46
Lactobacillus amylovorus GRL 1117 (LAB 31)	porcine jejunum [28]	SlpA	61
Lactobacillus amylovorus GRL 1117 (LAB 31)	porcine jejunum [28]	SlpB^b	49
Lactobacillus amylovorus GRL 1118 (LAB 52)	porcine jejunum [28]	SlpA	48
Escherichia coli F4⁺ (ETEC)	[26]	-^c	-^c
Escherichia coli ERF 2014; O141, F18⁺	DVB^d	-^c	-^c
Escherichia coli ATCC 43894; O157 (EHEC)	ATCC	-^c	-^c
Salmonella typhimurium ATCC14028	ATCC	-^c	-^c
Listeria monocytogenes R14-2-2	DVB^d	-^c	-^c
Yersinia enterocolitica R5-9-1	DVB^d	-^c	-^c
Escherichia coli DH5αF’	[31]	-^c	-^c
Escherichia coli BL21 (DE3)	EMD Millipore	-^c	-^c

^amolecular weight of mature protein ^bnot tested for adherence ^cnot present ^dculture collection of the Department of Veterinary Biosciences/Veterinary Microbiology and Epidemiology, University of Helsinki, Finland.

ISSN: 1471-2180