The Unculturables: targeted isolation of bacterial species associated with canine periodontal health or disease from dental plaque

Table 3 Bacterial species targeted using this approach and the media required to isolate them

Canine oral taxon	Species	Media	Atmosphere	Disease association
COT-005	Peptostreptococcaceae XI [G-1] sp.	HIA	Anaerobic	PD
COT-084	Odoribacter denticanis†	HIA	Anaerobic	PD
COT-107	Globicatella sp.	HIA	Aerobic	Health
COT-036	LachnospiraceaeXIVa [G-3] sp.	HIA	Anaerobic	PD
COT-044	Peptococcus sp. COT-044†	ASA	Anaerobic	PD
COT-064	Filifactor sp. ZP078	HIA	Anaerobic
COT-227	Peptostreptococcus sp.	HIA	Anaerobic
COT-388	Clostridiales III [G-3] sp.	HIA	Anaerobic	PD
COT-033	Peptostreptococcus sp.*	CBA H + M	Anaerobic
COT-016	Neisseria animaloris*	CBA	Aerobic
COT-192	Porphyromonas sp. IJD1952*	CBA H + M	Anaerobic
COT-108	Porphyromonas canoris*	CBA H + M	Anaerobic
COT-052	Porphyromonas gulae II*	CBA H + M	Anaerobic
COT-080	Pasteurellaceae [G-2] sp.*	CBA	Aerobic	Health
COT-029	Tissierella sp. COT-029	Not isolated	Anaerobic
COT-306	Chloroflexi sp. COT-306	Not isolated	Aerobic	PD
COT-280	Conchiformibius steedae COT-280	Not isolated	Aerobic

The canine oral taxon number relates to taxonomy assigned to species identified as part of a culture independent survey of the canine oral microbiome (Dewhirst et al.,[9]). †denotes the requirement of helper strain P. acnes and *denotes previously isolated species that were targeted for a study of intra-species diversity.

ISSN: 1471-2180