Characterization and regulation of the Resistance-Nodulation-Cell Division-type multidrug efflux pumps MdtABC and MdtUVW from the fire blight pathogen Erwinia amylovora

Table 4 Relative fold-changes of mdtA , mdtU , baeS and cpxR mRNA transcripts after inoculation of E. amylovora Ea1189 on apple rootstocks MM106 and immature pear fruit slices, respectively ^a

^aTotal RNA was isolated from bacterial cells recovered from infected plant tissues. Transcript abundance was determined by quantitative RT-PCR and compared to RT-PCR signals from cells grown in LB broth to an OD₆₀₀ of 0.5.
^bBacteria were re-isolated from infected shoots of apple rootstock 1, 4 and 7 days post inoculation (dpi).
^cBacteria were re-isolated from infected immature pears 12 hours post inoculation (hpi).
^dBoldface values indicate an increase of at least 2-fold.

ISSN: 1471-2180