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Figure 2 | BMC Microbiology

Figure 2

From: Regulated proteolysis of the alternative sigma factor SigX in Streptococcus mutans: implication in the escape from competence

Figure 2

Constitutive expression of MecA and its effect on the cellular levels of SigX. A. Western blot analysis of cellular levels of MecA in S. mutans strain GF-His2 (MecA-His) and strain GF-Ox (MecA-Ox) that constitutively expressed MecA. The samples were taken from the cultures of these strains grown during the early- (E), mid- (M) and late- (L) exponential phases to prepare the crude cell lysates. The cellular MecA of these strains were detected by Western blotting using the anti-His antibody. The intensities of the bands were scanned and converted as relative integrated density values (RIDV) for comparison. The total protein loading controls from the cell lysates of these strains were detected using the anti-S. mutans antibody. B. The effect of constitutively expressed MecA on the cellular levels of SigX in GF-Ox (MecA-Ox) grown in THYE in response to CSP. The cellular MecA was detected by Western blotting using the anti-His antibody, while the cellular SigX in the same strain was detected using the anti-SigX antibody.

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BMC Microbiology

ISSN: 1471-2180

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