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Figure 2 | BMC Microbiology

Figure 2

From: Host stress hormone norepinephrine stimulates pneumococcal growth, biofilm formation and virulence gene expression

Figure 2

Mechanism of norepinephrine growth induction. Panel A is a western blot of Tf binding to D39 cultures (+) compared with non-Tf supplemented control cultures (-); the influence of growth phase on Tf binding is also shown: log-phase (L), stationary phase (S). Tf represents purified transferrin and is the positive control, indicated by the arrow. Panel B shows that uptake of Tf-complexed Fe (in the form of 55Fe) is increased in the presence of NE, Pā€‰<ā€‰0.05; Panel C shows 3H-NE is internalised by D39. The values shown in panels B and C are means of triplicate counts; nā€‰=ā€‰3.

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