Figure 4
Lysozyme susceptibility assay. A) Lysozyme treatment growth curves for M. smegmatis/Rv1096 and wild-type M. smegmatis. M. smegmatis/Rv1096 (square) and wild-type M. smegmatis (triangle) were grown in LBT medium at 37°C to an OD600 of 0.2; the cultures were then divided into two parts. One part (closed symbol) was treated with lysozyme, the other part was not. Three microliter samples from each culture were collected at 1 h intervals for OD600 measurements. M. smegmatis/Rv1096 showed significantly greater resistance to lysozyme than did wild-type M. smegmatis (**P < 0.01). Values are means ± SD. B) Cell survival curves for M. smegmatis/Rv1096 and wild-type M. smegmatis under lysozyme treatment. M. smegmatis/Rv1096 (square) and wild-type M. smegmatis (triangle) were each grown in LBT medium at 37°C to an OD600 of 0.2, then the cultures were divided into two parts. One part (closed symbol) was treated with lysozyme, the other part was not. Three microliter culture samples were collected at 1 h intervals to measure CFU/ml. M. smegmatis/Rv1096 exhibited greater cell survival than that of the wild-type bacterium (**P < 0.01). Values are means ± SD.