From: The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen
Strain or plasmid | Relevant characteristics | Source or reference |
---|---|---|
Strains | ||
C. defragrans | [21] | |
65Phen-RIF | RaR | [27] |
ΔgeoB | 65Phen-RIF, RaR, ΔgeoB | This study |
ctmA::Tn5a | 65Phen-RIF, RaR, KmR, transposon insertion in ctmA at position 133 | This study |
ctmA::Tn5b | 65Phen-RIF, RaR, KmR, transposon insertion in ctmA at position 1188 | This study |
ctmB::Tn5a | 65Phen-RIF, RaR, KmR, transposon insertion in ctmB at position 38 | This study |
ctmB::Tn5b | 65Phen-RIF, RaR, KmR, transposon insertion in ctmB at position 559 | This study |
ctmE::Tn5 | 65Phen-RIF, RaR, KmR, transposon insertion in ctmE at position 111 | This study |
E. coli | ||
W20767 | RP4-2-tet::Mu-1, kan::Tn7 integrant, leu-63::IS10, recA1, creC510, hsdR17, endA1, zbf-5, uidA, (ΔMluI)::pir + thi | [37] |
S17-1 | Thi, pro, hsdR, recA with RP4-2[Tc::Mu-Km::Tn7] | [38] |
Plasmids | ||
pRL27 | Tn5 with KmR, R6K ori, oriT, RP4, tnp | [31] |
pCR4-TOPO | AmR, KmR, lacZα | Invitrogen |
pK19mobsacB | KmR, mob, sacB modified from B. subtilis, lacZα | [39] |
pK19mobsacBΔgeoB | KmR, mob, sacB modified from B. subtilis, lacZα, 2000b flanking regions of ΔgeoB | This study |