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Table 3 Bacterial strains and plasmids used in this work

From: The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen

Strain or plasmid Relevant characteristics Source or reference
Strains   
  C. defragrans   [21]
  65Phen-RIF RaR [27]
  ΔgeoB 65Phen-RIF, RaR, ΔgeoB This study
  ctmA::Tn5a 65Phen-RIF, RaR, KmR, transposon insertion in ctmA at position 133 This study
  ctmA::Tn5b 65Phen-RIF, RaR, KmR, transposon insertion in ctmA at position 1188 This study
  ctmB::Tn5a 65Phen-RIF, RaR, KmR, transposon insertion in ctmB at position 38 This study
  ctmB::Tn5b 65Phen-RIF, RaR, KmR, transposon insertion in ctmB at position 559 This study
  ctmE::Tn5 65Phen-RIF, RaR, KmR, transposon insertion in ctmE at position 111 This study
E. coli   
  W20767 RP4-2-tet::Mu-1, kan::Tn7 integrant, leu-63::IS10, recA1, creC510, hsdR17, endA1, zbf-5, uidA, (ΔMluI)::pir + thi [37]
  S17-1 Thi, pro, hsdR, recA with RP4-2[Tc::Mu-Km::Tn7] [38]
Plasmids   
  pRL27 Tn5 with KmR, R6K ori, oriT, RP4, tnp [31]
  pCR4-TOPO AmR, KmR, lacZα Invitrogen
  pK19mobsacB KmR, mob, sacB modified from B. subtilis, lacZα [39]
  pK19mobsacBΔgeoB KmR, mob, sacB modified from B. subtilis, lacZα, 2000b flanking regions of ΔgeoB This study