Bdellovibrio bacteriovorus HD100 guards against Pseudomonas tolaasii brown-blotch lesions on the surface of post-harvest Agaricus bisporus supermarket mushrooms

Table 3 Treatment conditions applied to mushroom pilei

Condition	Addition 1 (in 15 μl)	30 min, 21ËšC	Addition 2 (in 15 μl)	48 h, 29°C
King’s Medium B control	King’s Medium B broth	→	King’s Medium B broth	→
B. bacteriovorus alone	B. bacteriovorus HD100	→	King’s Medium B broth	→
P. tolaasii alone	P. tolaasii 2192^T	→	King’s Medium B broth	→
B. bacteriovorus before P. tolaasii	B. bacteriovorus HD100	→	P. tolaasii 2192^T	→
B. bacteriovorus after P. tolaasii	P. tolaasii 2192^T	→	B. bacteriovorus HD100	→

Details of the 5 pairwise combinations of B. bacteriovorus HD100, P. tolaasii 2192^T and King’s Medium B added to Agaricus bisporus mushrooms to test the effect of B. bacteriovorus predation of P. tolaasii on affected mushroom brown blotch lesion intensity.

ISSN: 1471-2180