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Table 2 Oligonucleotide sequences used to determine the minimal length of single-stranded and double-stranded DNA for DNA–Imu3 complex formation

From: The Escherichia coli uropathogenic-specific-protein-associated immunity protein 3 (Imu3) has nucleic acid -binding activity

Oligonucleotide

5′-3′ sequence

gCc

6-mer (M13)

gCggTTcv

67

7-mer (M13)

gCggTTC

63

8-mer (M13)

TggCggTT

63

9-mer (M13)

gTggCggTT

67

10-mer (M13)

ggTggCggTT

70

11-mer (M13)

ggTggCggTTC

73

12-mer (M13)

AgggTggCggTT

67

13-mer (M13)

gAgggTggCggTT

69

14-mer (M13)

gAgggTggCggTTC

71

15-mer (M13)

gAgggTggCggTTCT

67

15-TATA (poly AT)

TATATATATATATAT

0

15-gCgC (poly GC)

gCgCgCgCgCgCgCg

100

  1. The lengths of the oligonucleotides spanned from 6 to 32 nucleotides.
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BMC Microbiology

ISSN: 1471-2180

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