OmpU as a biomarker for rapid discrimination between toxigenic and epidemic Vibrio cholerae O1/O139 and non-epidemic Vibrio cholerae in a modified MALDI-TOF MS assay

Paauw, Armand; Trip, Hein; Niemcewicz, Marcin; Sellek, Ricela; Heng, Jonathan ME; Mars-Groenendijk, Roos H; de Jong, Ad L; Majchrzykiewicz-Koehorst, Joanna A; Olsen, Jaran S; Tsivtsivadze, Evgeni

doi:10.1186/1471-2180-14-158

Table 4 Results of BLASTp search using OmpU of Vibrio cholerae O1 El Tor N16961 (calculated molecular mass 34655.65 Da) as query sequence

From: OmpU as a biomarker for rapid discrimination between toxigenic and epidemic Vibrio cholerae O1/O139 and non-epidemic Vibrio cholerae in a modified MALDI-TOF MS assay

Hit nr.	Mutations compared to OmpU N16961	Theoretical mass (Da)	Strain	Serogroup	Serotype	Biotype	Origin	Year of isolation	ctxAB^a	tcpA^a	Epidemic (E) or non-epidemic strain (N)
1		34656	N16961	O1	Inaba	El tor	Bangladesh	1975	ctxAB+	tcpA+	E
1		34656	CP1032 (5)	O1	Ogawa	El tor	Mexico	1991	ctxAB+	tcpA+	E
1		34656	CP1044 (17)	O1^c			Peru	1991	ctxAB+	tcpA+	E
1		34656	4260B	O139			Bangladesh	1993	ctxAB+	tcpA+	E
1		34656	CP1046 (19)	O1^c			Peru	1995	ctxA+,ctxB^f	tcpA+	E
1		34656	CP1047 (20)	O1^c			Peru	1995	ctxAB+	tcpA+	E
1		34656	CP1033 (6)	O1^c			Mexico	2000	ctxAB+	tcpA+	E
1		34656	CIRS101	O1	Inaba	El tor	Bangladesh	2002	ctxAB+	tcpA+	E
1		34656	CP1037 (10)	O1			Mexico	2003	ctxA+,ctxB-^f	truncated	E
1		34656	CP1040 (13)	O1^c			Zambia	2004	ctxAB+	tcpA+	E
1		34656	CP1041 (14)	O1	Ogawa	El tor	Zambia	2004	ctxAB+	tcpA+	E
1		34656	CP1030 (3)	O1^c			Mexico	2008	ctxAB+	tcpA+	E
1		34656	HC-06A1 ^e	O1	Ogawa	El tor	Haiti	2010	ctxAB+	tcpA+	E
1		34656	CP1042 (15)	O1	Ogawa	El tor	Thailand	2010	ctxAB+	tcpA+	E
1		34656	CP1048 (21)	O1	Ogawa	El tor	Bangladesh	2010	ctxAB+	tcpA+	E
1		34656	CP1050 (23)	O1^c			Bangladesh	2010	ctxAB+	tcpA+	E
2^b		34656	M66-2	O1	-	-	Indonesia	1937	ctxA+,ctxB-^f	tcpA+	E
2		34656	MAK 757	O1	Ogawa	El Tor	Indonesia	1937	ctxAB+	tcpA+	E
2		34656	V52	O37			Sudan	1968	ctxAB+	tcpA+	E
2		34656	RC9	O1	Ogawa	El Tor	Kenya	1985	ctxAB+	tcpA+	E
2		34656	BX 330286	O1	Inaba	El Tor	Australia	1986	ctxAB+	tcpA+	E
2		34656	MO10	O139			India	1992	ctxAB+	tcpA+	E
2		34656	MJ-1236	O1	Inaba	El Tor	Bangladesh	1994	ctxAB+	tcpA+	E
2		34656	B33	O1	Ogawa	El Tor	Mozambique	2004	ctxAB+	tcpA+	E
3	F287I	34622	unknown	unknown		El tor			unknown	unknown	unknown
4	G325D	34714	CP1038 (11)	O1	Ogawa	El tor	Zimbabwe	2003	ctxAB+	tcpA+	E
5	E290K, V324A, 325S	34657	RC27	O1		Classical	Indonesia	1991	truncated	truncated	N
5	E290K, V324A, 325S	34657	O395	O1	Ogawa	Classical	India	1965	ctxAB+	truncated	N
7	10 mut	34598	BJG-01	non-O1^d					ctxA+,ctxB-^f	unknown	N
8	9 del , 13 mut	33840	HE-25	non-O1^d			Haiti	2010	ctxAB -	tcpA -	N
9	9 del, 13 mut	33840	AM-19226	O39			Bangladesh	2001	ctxAB -	tcpA -	N
10	7 del, 18 mut	33911	RC385	O135			USA	1998	ctxAB -	tcpA -	N

^a ctxAB and tcpA genes were identified by blastx search of whole genome sequences using ctxAB and tcpA of strain N16961 as query sequences.
^bHit nr. 2 represents OmpU identical to hit nr. 1 except for nine additional N-terminal residues resulting from a wrongly identified translation start.
^cPresumed serotype O1 based on sequence similarity with O-antigen biosynthesis genes VC0241 to VC0244A from N16961.
^dPresumed serotype non-O1/O139, based on lack of sequence similarity with O-antigen biosynthesis genes VC0241 to VC0244A from N16961 and O139. Accession: AB012956 bp 22084–24660 wbfH/wbfI/wbfJ ).
^eThis strain represents also 44 other Vibrio cholerae O1 El Tor Ogawa isolates from same outbreak with identical OmpU sequence and toxigenicity genes.
^fNo ctxB similar to ctxB of N16961 (locus_tag;VC1456). Presence of another variant of ctxB cannot be excluded.

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