Table 3 The primers used in PCR amplification and sequencing for confirmation of the mutation
Gene | Forward | Reverse | Annealing temperature |
|---|---|---|---|
dltA | AAGTAGTGCAGTTTAGGAGAGGA | AGATTGTACCACCGGATGTC | 58.0 |
gtcA | TTGAGCTCTTAGTAGAACCTGAC | CTGGTTTCGCTATCTCATTAG | 54.5 |
iap | CAAAATGCTACTACACACGCT | GTCAAAGAATACTAAATCACCAGC | 56.5 |