Figure 2

EV71 infection stimulates activation of JNK1/2 and p38 MAPK. (A and C) Western blot analysis of cell lysates of iDCs infected with EV71 at a MOI of 5 at 0 h, 1/2 h, 1 h, 2 h, 4 h, 8 h and 24 h p.i. using antibodies against total or phosphorylated JNK1/2, p38 MAPK, as well as internal control GAPDH. (B and D) Western blot analysis of cell lysates of iDCs preincubated with SP600125 and SB203580 (20 μM) for 1 h and infected with EV71 at a MOI of 5 at indicated times using antibodies against total and phosphorylated JNK1/2, p38 MAPK, as well as internal control GAPDH. The intensities of phosphorylated JNK1/2 and p38 MAPK were normalized to control level. The data were expressed as mean ± SE from three independent experiments and analyzed by one-way ANOVA (*p < 0.05, **P < 0.01 and ***P < 0.001).