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Table 3 Resistance genotypes, PBP3 types and PBP3 substitutions

From: Multilocus sequence typing and ftsI sequencing: a powerful tool for surveillance of penicillin-binding protein 3-mediated beta-lactam resistance in nontypeable Haemophilus influenzae

Resistance genotypesa PBP3 typesb n c Sgd Blae PBP3 substitutionsf
D S A M S P A I G A V R N A T V D A N
350 357 368 377 385 392 437 449 490 502 511 517 526 530 532 547 551 554 569
High-rPBP3                      
 Group III - 1    N N    T      T    K g    I    S
 Group III-like - 2    N N   I T        H    S I    
Low-rPBP3                      
 Group II A 48   1 N    I       V    K h    I    S
  B 19   5         V      K g    I    S
  C 5    N    I      E     K h    I    S
  D 17    N         E     K g S      
  F 1                K g       
  H 6             V    K h       
  I 4    N       S    V    K g    I    S
  J 3    N          T    K g    I    S
  K 2      T        T    K g       
  L 1    N         E     K g    I   Di S
  M 1    N          V    K h    I    S
  N 1   1 N       S V      K g    I    S
  O 1             T    K g    I    S
  P 1             T    K g    I    
  Q 1            E V    K h    I    S
 Group I - 2               H     I   T  
sPBP3 z0 51 15 6                    
z4 9 1   N                I    S
z1 7 3 2                 I    
z6 3                   I    S
z7 3                   I   T S
z5 1   1           S          
z8 1    N          T       I    S
z9 1    N                I    
z10 1                   I Ai   
z11 1              A      I    
z12 1         Si              
z13 1             T          
  1. aSee Table 1.
  2. bPBP3 types according to Skaare et al.[11] (types A – G) and this study (types H – Q and z0 – z13). ‘-‘, not designated.
  3. c n, No. of study isolates.
  4. dSg, No. of isolates from the Susceptible group included in n.
  5. eBla, No. of beta-lactamase positive isolates (all TEM-1) included in n.
  6. fAmino acid substitutions in PBP3 (transpeptidase domain, 338–573) with the amino acid sequence of H. influenzae Rd KW20 [GenBank:U32793] as reference (z0). Essential substitutions in bold.
  7. gN526K encoded by the DNA triplet AAG.
  8. hN526K encoded by the DNA triplet AAA.
  9. iNovel substitution.