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Table 3 Average values of different biofilm properties in the four selected media

From: On the influence of the culture conditions in bacterial antifouling bioassays and biofilm properties: Shewanella algae, a case study

Medium Mean thickness (μm) Max. thickness (μm) Coverage (%) Roughness coefficient Young modulus (MPa) Adhesion (nN)
MB 11.2 ± 0.8 25.3 ± 2.3 15.9 ± 1.7 1.92 ± 0.06 0.16 ± 0.10 1.33 ± 0.38
MH2 9.0 ± 1.2 13.5 ± 1.0 20.9 ± 2.4 0.97 ± 0.15 0.34 ± 0.16 0.73 ± 0.29
LMB 15.4 ± 2.2 20.5 ± 3.4 32.1 ± 4.6 0.65 ± 0.18 0.22 ± 0.13 0.85 ± 0.35
SASW 13.0 ± 0.8 29.5 ± 1.9 23.9 ± 3.9 1.40 ± 0.24 0.19 ± 0.09 1.11 ± 0.41
  1. Biofilm thickness (n = 12), surface coverage (n = 12) and roughness coefficients (n = 12) were determined from CLSM reconstructions. Young modulii and adhesion forces were quantified by AFM. In this case, at least 115 bacteria were individually analysed for each magnitude. Data represent the average ± SD.