On the influence of the culture conditions in bacterial antifouling bioassays and biofilm properties: Shewanella algae , a case study

Table 3 Average values of different biofilm properties in the four selected media

Medium	Mean thickness (μm)	Max. thickness (μm)	Coverage (%)	Roughness coefficient	Young modulus (MPa)	Adhesion (nN)
MB	11.2 ± 0.8	25.3 ± 2.3	15.9 ± 1.7	1.92 ± 0.06	0.16 ± 0.10	1.33 ± 0.38
MH2	9.0 ± 1.2	13.5 ± 1.0	20.9 ± 2.4	0.97 ± 0.15	0.34 ± 0.16	0.73 ± 0.29
LMB	15.4 ± 2.2	20.5 ± 3.4	32.1 ± 4.6	0.65 ± 0.18	0.22 ± 0.13	0.85 ± 0.35
SASW	13.0 ± 0.8	29.5 ± 1.9	23.9 ± 3.9	1.40 ± 0.24	0.19 ± 0.09	1.11 ± 0.41

Biofilm thickness (n = 12), surface coverage (n = 12) and roughness coefficients (n = 12) were determined from CLSM reconstructions. Young modulii and adhesion forces were quantified by AFM. In this case, at least 115 bacteria were individually analysed for each magnitude. Data represent the average ± SD.