Development of loop-mediated isothermal amplification assay for specific and rapid detection of differential goat Pox virus and Sheep Pox virus

Table 2 The detection sensitivity of reactions with different incubation temperatures and incubation times for each primer set

Sensitivity of reactions	Temperature option (incubation 60 min) (°C)				Time option (incubation at 60°C) (min)			Sensitivity (incubation at 60°C for 60 min) (copies/reaction)
Sensitivity of reactions	60	62	64	66	30	45	60	10⁹	10⁸	10⁷	10⁶	10⁵	10⁴	10³	10²	10¹	10°
GSPV Primer	+	+	+	-	-	+	+	+	+	+	+	+	+	+	-	-	-
GTPV Primer	+	+	+	-	-	-	+	+	+	+	+	-	-	-	-	-	-
SPPV Primer	+	+	+	+	+	+	+	+	+	+	+	+	+	-	-	-	-

Note: Optimization of incubation temperature and time for LAMP reactions when detecting about 100 ng of GTPV or SPPV genomic DNA using different primer sets. “+” stand for positive result and “-” stand for negative result.

ISSN: 1471-2180