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Table 2 The detection sensitivity of reactions with different incubation temperatures and incubation times for each primer set

From: Development of loop-mediated isothermal amplification assay for specific and rapid detection of differential goat Pox virus and Sheep Pox virus

Sensitivity of reactions Temperature option (incubation 60 min) (°C) Time option (incubation at 60°C) (min) Sensitivity (incubation at 60°C for 60 min) (copies/reaction)
60 62 64 66 30 45 60 109 108 107 106 105 104 103 102 101 10°
GSPV Primer + + + - - + + + + + + + + + - - -
GTPV Primer + + + - - - + + + + + - - - - - -
SPPV Primer + + + + + + + + + + + + + - - - -
  1. Note: Optimization of incubation temperature and time for LAMP reactions when detecting about 100 ng of GTPV or SPPV genomic DNA using different primer sets. “+” stand for positive result and “-” stand for negative result.