Genetic analysis of the roles of agaA, agaI, and agaS genes in the N-acetyl-D-galactosamine and D-galactosamine catabolic pathways in Escherichia colistrains O157:H7 and C

Table 1 Analysis of gene expression in EDL933, E. coli C, and their mutants by qRT-PCR

Carbon Source^a	Strain	Relative expression of genes in EDL933 and E. coli C^b
		agaA	agaS	nagA	nagB
Glycerol	EDL933/E. coli C	1/1	1/1	1/1	1/1
Aga	EDL933/E. coli C	375/32	495/62	1/1	1/1
GlcNAc	EDL933/E. coli C	1/3	1/3	12/16	24/23
Glycerol	EDL933 ∆agaA /E. coli C ∆agaA	ND/ND^c	1/1	1/1	1/1
Aga	EDL933 ∆agaA /E. coli C ∆agaA	ND/ND	699/86	16/7	28/9
GlcNAc	EDL933 ∆agaA /E. coli C ∆agaA	ND/ND	5/3	12/9	20/13
Glycerol	EDL933∆nagA /E. coli C ∆nagA	2/0.5	2/0.2	ND/ND	61/19
Aga	EDL933∆nagA /E. coli C ∆nagA	820/179	917/93	ND/ND	8/2

^a Carbon source used for growth.
^b The relative expression values after the forward slash is that of E. coli C.
^c ND indicates not detected.

ISSN: 1471-2180