Impact of agr dysfunction on virulence profiles and infections associated with a novel methicillin-resistant Staphylococcus aureus (MRSA) variant of the lineage ST1-SCCmec IV

Table 1 Primers used in Real Time qPCR

Target gene	Primer sequence^a	Amplicon length (bp)	Reference
rnaIII	F: AATTTGTTCACTGTGTCGATAAT	135	This study
rnaIII	R:TGGAAAATAGTTGATGAGTTGTT	135	This study
sarA	F: TTCTTTCTCTTTGTTTTCGCTG	115	This study
sarA	R: GTTATCAATGGTCACTTATGCT	115	This study
spa	F: TGGTTTGCTGGTTGCTTCTTA	116	This study
spa	R: GCAAAAGCAAACGGCACTAC	116	This study
hla	F: TTTGTCATTTCTTCTTTTTCCCA	169	This study
hla	R: AAGCATCCAAACAACAAACAAAT	169	This study
psmα	F:TATCAAAAGCTTAATCGAACAATTC	176	53
psmα	R: CCCCTTCAAATAAGATGTTCATATC	176	53
sasG	F:GGTTTTCAGGTCCTTTTGGAT	192	This study
sasG	R:CTGGTGAAGAGCGAGTGAAA	192	This study
fnbpA	F: ACTTGATTTTGTGTAGCCTTTTT	185	This study
fnbpA	R:GAAGAAGCACCAAAAGCAGTA	185	This study
fnbpB	F:CGTTATTTGTAGTTGTTTGTGTT	118	This study
fnbpB	R:TGGAATGGGACAAGAAAAAGAA	118	This study
rrna 16S	F: AGAGATAGAGCCTTCCCCTT	84	This study
rrna 16S	R:TTAACCCAACATCTCACGACA	84	This study
mecA	F:TCCAGATTACAACTTCACCAGG	162	54
	R:CCACTTCATATCTTGTAACG

^aF and R: forward and reverse primers, respectively, in 5´→ 3´orientation. The cycling conditions for all primers were as follows: One cycle of 48°C/30min and 95°C/10 min, followed by 35 cycles of 95°C/30s, 55°C/45s and 72°C/45 s. Each run included a nontemplate and a gene-negative RNA controls.

ISSN: 1471-2180