Table 5 PCR conditions used for detection of antimicrobial resistance genes
Resistance | Gene | Primers | Tm (°C) | Amplicon size (pb) |
|---|---|---|---|---|
β-Lactams | bla TEM-1 | 5′-ttgggtgcacgagtgggt-3′ | 60 | 503 |
5′-taattgttgccgggaagc-3′ | ||||
bla oxa-1 | 5′-agcagcgccagtgcatca-3′ | 708 | ||
5′-attcgaccccaagtttcc-3′ | ||||
bla pse-1 | 5′-cgcttcccgttaacaagtac-3′ | 419 | ||
5′-ctggttcatttcagatagcg-3′ | ||||
bla shv | 5′-tcgggccgcgtaggcatg-3′ | 606 | ||
5′-agcagggcgacaatcccg-3′ | ||||
Quinolones | qnrA | 5′-agaggatttctcacgccagg-3′ | 56 | 579 |
5′-tgccaggcacagatcttgac-3′ | ||||
qnrB | 5′-ggmathgaaattcgccactg-3′ | 263 | ||
5′-ttygcbgyycgccagtcggcg-3′ | ||||
qnrS | 5′-gcaagttcattgaacagggt-3′ | 427 | ||
5′-tctaaaccgtcgagttcggcg-3′ | ||||
qepA | 5′-aactgcttgagcccgtagat-3′ | 189 | ||
5′-cgtgttgctggagttcttcc-3′ | ||||
aac(6′)-Ib-cr | 5′-ttgcaatgctgaatggagag-3′ | 218 | ||
5′-cgtttggatcttggtgacct-3′ | ||||
Cephalosporins | bla ctx-m-like | 5′-cgatgtgcagtaccagtaa-3′ | 60 | 585 |
5′-ttagtgaccagaatcagcgg-3′ | ||||
bla ctx-m-group1 | 5′-atggttaaaaaatcactgcg-3′ | 876 | ||
5′-ttacaaaccgtcggtgac-3′ | ||||
bla ctx-m-group9 | 5′-atggtgacaaagagagtgcaac-3′ | 876 | ||
5′-ttacagcccttcggcgatg-3′ | ||||
bla ctx-m-group2 | 5′-tcaagaagagcgacctggtt-3′ | 601 | ||
5′-gatacctcgctccatttattg-3′ | ||||
Cephamycinases | bla acc | 5′-aacagcctcagcagccggtta-3′ | 64 | 346 |
5′-ttcgccgcaatcatccctagc-3′ | ||||
bla cit | 5′-tggccagaactgacaggcaaa-3′ | 462 | ||
5′-tttctcctgaacgtggctggc-3′ | ||||
bla dha | 5′-aactttcacaggtgtgctgggt-3′ | 405 | ||
5′-ccgtacgcatactggctttgc-3′ | ||||
bla mox | 5′-gctgctcaaggagcacaggat-3′ | 520 | ||
5′-cacattgacataggtgtggtg-3′ | ||||
bla ebc | 5′-tcggtaaagccgatgttgcgg-3′ | 302 | ||
5′-cttccactgcggctgccagtt-3′ | ||||
bla fox | 5′-aacatggggtatcagggagatg-3′ | 190 | ||
5′-caaagcgcgtaaccggattgg-3′ | ||||
Tetracyclines | tet (G) | 5′-gctcggtggtatctctgc-3′ | 60 | 500 |
5′-agcaacagaatcgggaac-3′ | ||||
tet (A) | 5′-gctacatcctgcttgcct-3′ | 210 | ||
5′-catagatcgccgtgaaga-3′ | ||||
tet (B) | 5′-ttggttaggggcaagttttg-3′ | 600 | ||
5′-gtaatgggccaataacaccg-3′ | ||||
Integrons | intI1 | 5′-gccttgctgttcttctac-3′ | 55 | 558 |
5′-gatgcctgcttgttctac-3′ | ||||
intI2 | 5′-cacggatatgcgacaaaaaggt-3′ | 65 | 788 | |
5′-gtagcaaacgagtgacgaaatg-3′ | ||||
Int-class 1 (IC1) | 5′-ggcatccaagcagcaagc-3′ | 58 | variable | |
5′-aagcagacttgacctgat-3′ | ||||
Int-class 2 (IC2) | 5′-cgggatcccggacggcatgcacgatttgta-3′ | 65 | variable | |
5′-gatgccatcgcaagtacgag-3′ |