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Table 5 PCR conditions used for detection of antimicrobial resistance genes

From: Molecular characterization of multiresistant Escherichia coli producing or not extended-spectrum β-lactamases

Resistance Gene Primers Tm (°C) Amplicon size (pb)
β-Lactams bla TEM-1 5′-ttgggtgcacgagtgggt-3′ 60 503
5′-taattgttgccgggaagc-3′
bla oxa-1 5′-agcagcgccagtgcatca-3′ 708
5′-attcgaccccaagtttcc-3′
bla pse-1 5′-cgcttcccgttaacaagtac-3′ 419
5′-ctggttcatttcagatagcg-3′
bla shv 5′-tcgggccgcgtaggcatg-3′ 606
5′-agcagggcgacaatcccg-3′
Quinolones qnrA 5′-agaggatttctcacgccagg-3′ 56 579
5′-tgccaggcacagatcttgac-3′
qnrB 5′-ggmathgaaattcgccactg-3′ 263
5′-ttygcbgyycgccagtcggcg-3′
qnrS 5′-gcaagttcattgaacagggt-3′ 427
5′-tctaaaccgtcgagttcggcg-3′
qepA 5′-aactgcttgagcccgtagat-3′ 189
5′-cgtgttgctggagttcttcc-3′
aac(6′)-Ib-cr 5′-ttgcaatgctgaatggagag-3′ 218
5′-cgtttggatcttggtgacct-3′
Cephalosporins bla ctx-m-like 5′-cgatgtgcagtaccagtaa-3′ 60 585
5′-ttagtgaccagaatcagcgg-3′
bla ctx-m-group1 5′-atggttaaaaaatcactgcg-3′ 876
5′-ttacaaaccgtcggtgac-3′
bla ctx-m-group9 5′-atggtgacaaagagagtgcaac-3′ 876
5′-ttacagcccttcggcgatg-3′
bla ctx-m-group2 5′-tcaagaagagcgacctggtt-3′ 601
5′-gatacctcgctccatttattg-3′
Cephamycinases bla acc 5′-aacagcctcagcagccggtta-3′ 64 346
5′-ttcgccgcaatcatccctagc-3′
bla cit 5′-tggccagaactgacaggcaaa-3′ 462
5′-tttctcctgaacgtggctggc-3′
bla dha 5′-aactttcacaggtgtgctgggt-3′ 405
5′-ccgtacgcatactggctttgc-3′
bla mox 5′-gctgctcaaggagcacaggat-3′ 520
5′-cacattgacataggtgtggtg-3′
bla ebc 5′-tcggtaaagccgatgttgcgg-3′ 302
5′-cttccactgcggctgccagtt-3′
bla fox 5′-aacatggggtatcagggagatg-3′ 190
5′-caaagcgcgtaaccggattgg-3′
Tetracyclines tet (G) 5′-gctcggtggtatctctgc-3′ 60 500
5′-agcaacagaatcgggaac-3′
tet (A) 5′-gctacatcctgcttgcct-3′ 210
5′-catagatcgccgtgaaga-3′
tet (B) 5′-ttggttaggggcaagttttg-3′ 600
5′-gtaatgggccaataacaccg-3′
Integrons intI1 5′-gccttgctgttcttctac-3′ 55 558
5′-gatgcctgcttgttctac-3′
intI2 5′-cacggatatgcgacaaaaaggt-3′ 65 788
5′-gtagcaaacgagtgacgaaatg-3′
Int-class 1 (IC1) 5′-ggcatccaagcagcaagc-3′ 58 variable
5′-aagcagacttgacctgat-3′
Int-class 2 (IC2) 5′-cgggatcccggacggcatgcacgatttgta-3′ 65 variable
   5′-gatgccatcgcaagtacgag-3′