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Table 1 Average melting temperature of reference amplicons with CV% and SDs

From: Rapid plasmid replicon typing by real time PCR melting curve analysis

Replicon name Size of reference plasmid and amplicon (bp) Melting temperature of amplicon (°C) Average TM SD CV%
A/C 4365 86.3 86.3 0.05 0.06
B/O 4059 85.1 85.1 0.17 0.20
ColE 4087 86.4 86.4 0.20 0.23
ColEtp 4006 84.9 84.9 0.13 0.16
F 4170 84.2 84.2 0.24 0.29
FIA 4362 84.0 84 0.17 0.21
FIB 4602 86.4 86.4 0.07 0.08
FIC 4162 83.6 83.6 0.15 0.18
FIIs 4170 87.7 87.7 0.18 0.20
HI1 4371 83.6 83.6 0.18 0.21
HI2 4544 86.3 86.3 0.11 0.13
I1 4039 83.3 83.3 0.12 0.15
K 4060 85.2 85.2 0.09 0.10
L/M 4685 84.7 84.7 0.08 0.10
N 4459 86.5 86.5 0.17 0.19
P 4434 88.4 88.4 0.15 0.17
R 4151 84.4 84.4 0.18 0.21
T 4650 83.8 83.8 0.19 0.23
U 4743 88.9 88.9 0.09 0.10
W 4142 88.9 88.9 0.09 0.10
X 4276 82.1 82.1 0.22 0.27
Y 4665 86.6 86.6 0.31 0.36
  1. Reference plasmids, sizes and average melting temperatures obtained from at least five crude lysates of the cloned replicon plasmid. The average melting temperatures for replicons from WT strains were identical to those of the cloned replicons.