Rapid plasmid replicon typing by real time PCR melting curve analysis

BMC Microbiology

Table 1 Average melting temperature of reference amplicons with CV% and SDs

Replicon name	Size of reference plasmid and amplicon (bp)	Melting temperature of amplicon (°C)	Average TM	SD	CV%
A/C	4365	86.3	86.3	0.05	0.06
B/O	4059	85.1	85.1	0.17	0.20
ColE	4087	86.4	86.4	0.20	0.23
ColEtp	4006	84.9	84.9	0.13	0.16
F	4170	84.2	84.2	0.24	0.29
FIA	4362	84.0	84	0.17	0.21
FIB	4602	86.4	86.4	0.07	0.08
FIC	4162	83.6	83.6	0.15	0.18
FIIs	4170	87.7	87.7	0.18	0.20
HI1	4371	83.6	83.6	0.18	0.21
HI2	4544	86.3	86.3	0.11	0.13
I1	4039	83.3	83.3	0.12	0.15
K	4060	85.2	85.2	0.09	0.10
L/M	4685	84.7	84.7	0.08	0.10
N	4459	86.5	86.5	0.17	0.19
P	4434	88.4	88.4	0.15	0.17
R	4151	84.4	84.4	0.18	0.21
T	4650	83.8	83.8	0.19	0.23
U	4743	88.9	88.9	0.09	0.10
W	4142	88.9	88.9	0.09	0.10
X	4276	82.1	82.1	0.22	0.27
Y	4665	86.6	86.6	0.31	0.36

Reference plasmids, sizes and average melting temperatures obtained from at least five crude lysates of the cloned replicon plasmid. The average melting temperatures for replicons from WT strains were identical to those of the cloned replicons.

ISSN: 1471-2180