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Table 3 Results of the Lac663 and Gard162 probes specificity test in artificial mixed samples

From: Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.

Species in the artificial mixed samples

Bacteria strain collection codes

Multiplex PNA-FISH assay

Lac663 Probe efficiency

Gard162 Probe efficiency

L. pentosus;

CECT 4023T; -

++++

++++

G. vaginalis 51

L. casei;

CECT 5275T; -

++++

++++

G. vaginalis 101

L. rhamnosus;

CECT 288T; -

++++

++++

G. vaginalis AMD

L. crispatus;

ATCC 33820T; -

++++

++++

G. vaginalis ATCC

L. delbrueckii sub. delbrueckii; Atopobium vaginae

ATCC 9649T; CCUG 38953T

+++

-

L. acidophilus;

ATCC 4356T; CCUG 42099T

++++

-

A. vaginae

L. gasseri;

ATCC 9857T; CCUG 44116T

++++

-

A. vaginae

L. paracasei sub. paracasei;

CCUG 27320T; -

+++

−/+

L. lactis 53

L. rhamnosus;

ATCC 7469T; CECT 410T

++++

-

E. faecium

L. reuteri;

NCFB 2656T;

+++

-

E. coli O157:H7

NCTC 12900T

S. aureus;

CECT 976T; -

-

++++

G. vaginalis 51

Shigella;

ATCC 12022T; -

-

++++

G. vaginalis 101

L. seeligeri;

CECT 917T; -

-

++++

G. vaginalis AMD

E. aerogenes;

CECT 684T; -

-

++++

G. vaginalis ATCC

L. pentosus;

CECT 4023T;

++++

++++

G. vaginalis ATCC;

-;

E. faecalis

CECT 184T

L. casei;

CECT 5275T;

++++

++++

G. vaginalis AMD;

-;

A. vaginae

CCUG 38953T

L. rhamnosus;

CECT 288T;

++++

++++

G. vaginalis 101;

-;

A. vaginae

CCUG 42099T

L. crispatus;

ATCC 33820T;

++++

++++

G. vaginalis 51;

-;

A. vaginae

CCUG 44116T

L. casei;

CECT 5275T;

++++

-

L. mesenteroides;

-;

A. vaginae

CCUG 38953T

  1. The PNA probe (Lac663 and Gar162) efficiencies were tested in triplicate experiments for each strain, with the following hybridization PNA FISH qualitative evaluation: (−) Absence of hybridization; (+) Poor hybridization; (+++) Good hybridization; (++++) Optimal hybridization. Median values from the three experiments for each strain are shown in the table.