Species in the artificial mixed samples
|
Bacteria strain collection codes
|
Multiplex PNA-FISH assay
|
---|
Lac663 Probe efficiency
|
Gard162 Probe efficiency
|
---|
L. pentosus;
|
CECT 4023T; -
|
++++
|
++++
|
G. vaginalis 51
|
L. casei;
|
CECT 5275T; -
|
++++
|
++++
|
G. vaginalis 101
|
L. rhamnosus;
|
CECT 288T; -
|
++++
|
++++
|
G. vaginalis AMD
|
L. crispatus;
|
ATCC 33820T; -
|
++++
|
++++
|
G. vaginalis ATCC
|
L. delbrueckii sub. delbrueckii; Atopobium vaginae
|
ATCC 9649T; CCUG 38953T
|
+++
|
-
|
L. acidophilus;
|
ATCC 4356T; CCUG 42099T
|
++++
|
-
|
A. vaginae
|
L. gasseri;
|
ATCC 9857T; CCUG 44116T
|
++++
|
-
|
A. vaginae
|
L. paracasei sub. paracasei;
|
CCUG 27320T; -
|
+++
|
−/+
|
L. lactis 53
|
L. rhamnosus;
|
ATCC 7469T; CECT 410T
|
++++
|
-
|
E. faecium
|
L. reuteri;
|
NCFB 2656T;
|
+++
|
-
|
E. coli O157:H7
|
NCTC 12900T
|
S. aureus;
|
CECT 976T; -
|
-
|
++++
|
G. vaginalis 51
|
Shigella;
|
ATCC 12022T; -
|
-
|
++++
|
G. vaginalis 101
|
L. seeligeri;
|
CECT 917T; -
|
-
|
++++
|
G. vaginalis AMD
|
E. aerogenes;
|
CECT 684T; -
|
-
|
++++
|
G. vaginalis ATCC
|
L. pentosus;
|
CECT 4023T;
|
++++
|
++++
|
G. vaginalis ATCC;
|
-;
|
E. faecalis
|
CECT 184T
|
L. casei;
|
CECT 5275T;
|
++++
|
++++
|
G. vaginalis AMD;
|
-;
|
A. vaginae
|
CCUG 38953T
|
L. rhamnosus;
|
CECT 288T;
|
++++
|
++++
|
G. vaginalis 101;
|
-;
|
A. vaginae
|
CCUG 42099T
|
L. crispatus;
|
ATCC 33820T;
|
++++
|
++++
|
G. vaginalis 51;
|
-;
|
A. vaginae
|
CCUG 44116T
|
L. casei;
|
CECT 5275T;
|
++++
|
-
|
L. mesenteroides;
|
-;
|
A. vaginae
|
CCUG 38953T
|
- The PNA probe (Lac663 and Gar162) efficiencies were tested in triplicate experiments for each strain, with the following hybridization PNA FISH qualitative evaluation: (−) Absence of hybridization; (+) Poor hybridization; (+++) Good hybridization; (++++) Optimal hybridization. Median values from the three experiments for each strain are shown in the table.