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Table 3 Results of the Lac663 and Gard162 probes specificity test in artificial mixed samples

From: Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.

Species in the artificial mixed samples Bacteria strain collection codes Multiplex PNA-FISH assay
Lac663 Probe efficiency Gard162 Probe efficiency
L. pentosus; CECT 4023T; - ++++ ++++
G. vaginalis 51
L. casei; CECT 5275T; - ++++ ++++
G. vaginalis 101
L. rhamnosus; CECT 288T; - ++++ ++++
G. vaginalis AMD
L. crispatus; ATCC 33820T; - ++++ ++++
G. vaginalis ATCC
L. delbrueckii sub. delbrueckii; Atopobium vaginae ATCC 9649T; CCUG 38953T +++ -
L. acidophilus; ATCC 4356T; CCUG 42099T ++++ -
A. vaginae
L. gasseri; ATCC 9857T; CCUG 44116T ++++ -
A. vaginae
L. paracasei sub. paracasei; CCUG 27320T; - +++ −/+
L. lactis 53
L. rhamnosus; ATCC 7469T; CECT 410T ++++ -
E. faecium
L. reuteri; NCFB 2656T; +++ -
E. coli O157:H7 NCTC 12900T
S. aureus; CECT 976T; - - ++++
G. vaginalis 51
Shigella; ATCC 12022T; - - ++++
G. vaginalis 101
L. seeligeri; CECT 917T; - - ++++
G. vaginalis AMD
E. aerogenes; CECT 684T; - - ++++
G. vaginalis ATCC
L. pentosus; CECT 4023T; ++++ ++++
G. vaginalis ATCC; -;
E. faecalis CECT 184T
L. casei; CECT 5275T; ++++ ++++
G. vaginalis AMD; -;
A. vaginae CCUG 38953T
L. rhamnosus; CECT 288T; ++++ ++++
G. vaginalis 101; -;
A. vaginae CCUG 42099T
L. crispatus; ATCC 33820T; ++++ ++++
G. vaginalis 51; -;
A. vaginae CCUG 44116T
L. casei; CECT 5275T; ++++ -
L. mesenteroides; -;
A. vaginae CCUG 38953T
  1. The PNA probe (Lac663 and Gar162) efficiencies were tested in triplicate experiments for each strain, with the following hybridization PNA FISH qualitative evaluation: (−) Absence of hybridization; (+) Poor hybridization; (+++) Good hybridization; (++++) Optimal hybridization. Median values from the three experiments for each strain are shown in the table.