Probe
|
Type
|
Sequence (5´→3´)
|
No. of Lactobacillus strains detected a
|
No. of non- Lactobacillus strains detected a
|
Specificity (%)a
|
Sensibility (%)a
|
Reference or source
|
---|
Lab158b
|
DNA
|
GGTATTAGCA(C/T)CTGTTTCCA
|
11,991
|
7,165
|
99.30g
|
92.69 g
|
[28]
|
LGC354Ac
|
DNA
|
TGGAAGATTCCCTACTGC
|
12,701
|
12,329
|
98.79 g
|
98.18 g
|
[29]
|
LAB759e
|
DNA
|
CTACCCATRCTTTCGAGCC
|
10,371
|
2,823
|
99.72 g
|
80.17 g
|
[30]
|
Name not available
|
PNA
|
CCATTGTGGAAGATTC
|
12,930
|
14,880
|
98.54 g
|
99.95 g
|
[31]
|
Lac663
|
PNA
|
ACATGGAGTTCCACT
|
11,837
|
3,548
|
99.65 g
|
91.50 g
|
[26]
|
GardV
|
DNA
|
CCACCGTTACACCGAGAA
|
20
|
39
|
99.99
|
50.00
|
[10]
|
G.vag1008f
|
DNA
|
CTGCAGAGATGTGGTTTCCYTTCG
|
39
|
7
|
100.00
|
97.50
|
[32]
|
G.vag198
|
DNA
|
CCACTAAACACTTTCCCAACAAGA
|
34
|
0
|
100.00
|
85.00
|
[6]
|
GV003
|
DNA
|
AGACGGCTCCATCCCAAAAGGGTT
|
32
|
0
|
100.00
|
80.00
|
[33]
|
Gard162
|
PNA
|
CAGCATTACCACCCG
|
38
|
1
|
100.00
|
95.00
|
This work
|
- a Calculated through ProbeMatch/, last accession, May 2012) with the following data set options: Strain – Both; Source – Both; Size – > 1200 bp; Quality – Both.
- b DNA probe that also detects members of Enterococcus, Pediococcus, Weissella, Vagococcus, Leuconostoc and Oenococcus spp. used by Lebeer et al. [34].
- c DNA probe mainly detecting members of Lactobacillales and Bacillales, such as Lactobacillus spp., used in Olsen et al. [35].
- e DNA probe also detects members of Ruminococcaceae sp. and Pediococcus sp. used in Quevedo et al. [36]; The R symbol of the DNA probe sequence may be Adenosine or Guanosine, therefore Quevedo et al. [36] used a degenerate base in the sequence of the DNA probe to detect Lactobacillus spp.
- f The Y symbol of the DNA probe sequence may be Cytidine or Thymidine, therefore Fredricks et al. [6] used a degenerate base in the sequence of the DNA probe to detect G. vaginalis
- g Values determined in Machado et al.[26].