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Table 1 Bacterial strains used in PNA-FISH assays and their specificity with Lac663 and Gard162 probes

From: Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.

Bacterial species Collection strain Lac663 Probe efficiency Gard162 Probe efficiency
Lactobacillus acidophilus ATCC 4356T ++++ -
L. crispatus ATCC 33820T ++++ -
L. gasseri ATCC 9857T ++++ -
L. reuteri NCFB 2656T +++ -
L. rhamnosus ATCC 7469T ++++ -
L. rhamnosus CECT 288T ++++ -
L. johnsonii ATCC 11506T ++++ -
L. hilgardii NCFB 962T +++ -
L. delbrueckii subsp. delbrueckii ATCC 9649T +++ -
L. delbrueckii subsp. lactis ATCC 12315T +++ -
L. pentosus CECT 4023T ++++ -
L. casei CECT 5275T ++++ -
L. coryniformis subsp. torquens CECT 4129T ++++ -
L. paracasei CECT 227T ++++ -
L. agilis CCUG 31450T ++++ -
L. animalis ATCC 35046T +++ -
L. bifermentans ATCC 35409T +++ -
L. brevis ATCC 14869T ++++ -
L. buchneri ATCC 4005T +++ -
L. fermentum ATCC 11739T +++ -
L. curvatus subsp. curvatus ATCC 25601T ++++ -
L. farciminis DSM 20182T ++++ -
L. fructivorans ATCC 8288T +++ -
L. gallinarum CCUG 31412T ++++ -
L. graminis DSM 20719T ++ -
L. hamsteri ATCC 43851T +++ -
L. helveticus ATCC 15009T ++++ -
L. intestinalis ATCC 49335T +++ -
L. murinus ATCC 35020T ++++ -
L. parabuchneri ATCC 12936T ++++ -
L. paracasei subsp. paracasei CCUG 27320T +++ -
L. plantarum NCIMB 8827T +++ -
L. ruminis ATCC 27781T ++++ -
L. sakei subsp. carnosus CCUG 8045T ++ -
L. salivarius DEVRIESE 94/438T +++ -
L. plantarum NCCB 46043T +++ -
L. lactis 53 - - -
Streptococcus. thermophilus A - - -
S. thermophilus B - +++ -
Leuconostoc mesenteroides - - -
Bacillus subtilis DSM 7-10T - -
Enterococcus faecium CECT 410T - -
E. faecalis CECT 184T - -
Gardnerella vaginalis 5-1 - - ++++
G. vaginalis 101 - - ++++
G. vaginalis AMD - - ++++
G. vaginalis ATCC - ++++
G. vaginalis Belgian isolate 1 - +++
G. vaginalis Belgian isolate 2 - ++++
G. vaginalis Belgian isolate 3 - ++++
G. vaginalis Belgian isolate 4 - ++++
G. vaginalis Belgian isolate 5 - ++++
G. vaginalis Belgian isolate 6 - ++++
G. vaginalis Belgian isolate 7 - +++
G. vaginalis Belgian isolate 8 - +++
G. vaginalis Belgian isolate 9 - ++++
G. vaginalis Belgian isolate 10 - ++
G. vaginalis Belgian isolate 11 - ++++
G. vaginalis Belgian isolate 12 - +++
G. vaginalis Belgian isolate 13 - +++
G. vaginalis Belgian isolate 14 - ++
G. vaginalis Belgian isolate 15 - +++
G. vaginalis Belgian isolate 16 - +++
G. vaginalis Belgian isolate 17 - ++++
G. vaginalis Belgian isolate 18 - ++++
Atopobium vaginae CCUG 38953T - -
A. vaginae CCUG 42099T - -
A. vaginae CCUG 44116T - -
A. vaginae Clinical isolate - -
Bacillus cereus - - -
Enterobacter aerogenes CECT 684T - -
Escherichia coli O157:H7 NCTC 12900T - -
Staphylococcus aureus CECT 976T - -
S. aureus CECT 86T - -
Shigella flexneri ATCC 12022T - -
Listeria monocytogenes - - -
L. monocytogenes CECT 5873T - -
L. seeligeri CECT 917T - -
Klebsiella pneumoniae subsp. ozaenae ATCC 11296T - -
Salmonella Typhi - - -
S. enterica - - -
Escherichia coli CECT 434T - -
Prevotella bivia ATCC 29303T - -
Mobiluncus mulieris ATCC 26-9T - -
Fusobacteria nucleatum Clinical isolate - -
  1. The PNA Probe (Lac663 and Gar162) efficiencies were tested in triplicate experiments for each strain, with the following hybridization PNA FISH qualitative evaluation: (−) Absence of hybridization; (++) Moderate hybridization; (+++) Good hybridization; (++++) Optimal hybridization. The table shows the median value from the three experiments for each strain.