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Table 1 Bacterial strains used in PNA-FISH assays and their specificity with Lac663 and Gard162 probes

From: Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.

Bacterial species

Collection strain

Lac663 Probe efficiency

Gard162 Probe efficiency

Lactobacillus acidophilus

ATCC 4356T

++++

-

L. crispatus

ATCC 33820T

++++

-

L. gasseri

ATCC 9857T

++++

-

L. reuteri

NCFB 2656T

+++

-

L. rhamnosus

ATCC 7469T

++++

-

L. rhamnosus

CECT 288T

++++

-

L. johnsonii

ATCC 11506T

++++

-

L. hilgardii

NCFB 962T

+++

-

L. delbrueckii subsp. delbrueckii

ATCC 9649T

+++

-

L. delbrueckii subsp. lactis

ATCC 12315T

+++

-

L. pentosus

CECT 4023T

++++

-

L. casei

CECT 5275T

++++

-

L. coryniformis subsp. torquens

CECT 4129T

++++

-

L. paracasei

CECT 227T

++++

-

L. agilis

CCUG 31450T

++++

-

L. animalis

ATCC 35046T

+++

-

L. bifermentans

ATCC 35409T

+++

-

L. brevis

ATCC 14869T

++++

-

L. buchneri

ATCC 4005T

+++

-

L. fermentum

ATCC 11739T

+++

-

L. curvatus subsp. curvatus

ATCC 25601T

++++

-

L. farciminis

DSM 20182T

++++

-

L. fructivorans

ATCC 8288T

+++

-

L. gallinarum

CCUG 31412T

++++

-

L. graminis

DSM 20719T

++

-

L. hamsteri

ATCC 43851T

+++

-

L. helveticus

ATCC 15009T

++++

-

L. intestinalis

ATCC 49335T

+++

-

L. murinus

ATCC 35020T

++++

-

L. parabuchneri

ATCC 12936T

++++

-

L. paracasei subsp. paracasei

CCUG 27320T

+++

-

L. plantarum

NCIMB 8827T

+++

-

L. ruminis

ATCC 27781T

++++

-

L. sakei subsp. carnosus

CCUG 8045T

++

-

L. salivarius

DEVRIESE 94/438T

+++

-

L. plantarum

NCCB 46043T

+++

-

L. lactis 53

-

-

-

Streptococcus. thermophilus A

-

-

-

S. thermophilus B

-

+++

-

Leuconostoc mesenteroides

-

-

-

Bacillus subtilis

DSM 7-10T

-

-

Enterococcus faecium

CECT 410T

-

-

E. faecalis

CECT 184T

-

-

Gardnerella vaginalis 5-1

-

-

++++

G. vaginalis 101

-

-

++++

G. vaginalis AMD

-

-

++++

G. vaginalis

ATCC

-

++++

G. vaginalis

Belgian isolate 1

-

+++

G. vaginalis

Belgian isolate 2

-

++++

G. vaginalis

Belgian isolate 3

-

++++

G. vaginalis

Belgian isolate 4

-

++++

G. vaginalis

Belgian isolate 5

-

++++

G. vaginalis

Belgian isolate 6

-

++++

G. vaginalis

Belgian isolate 7

-

+++

G. vaginalis

Belgian isolate 8

-

+++

G. vaginalis

Belgian isolate 9

-

++++

G. vaginalis

Belgian isolate 10

-

++

G. vaginalis

Belgian isolate 11

-

++++

G. vaginalis

Belgian isolate 12

-

+++

G. vaginalis

Belgian isolate 13

-

+++

G. vaginalis

Belgian isolate 14

-

++

G. vaginalis

Belgian isolate 15

-

+++

G. vaginalis

Belgian isolate 16

-

+++

G. vaginalis

Belgian isolate 17

-

++++

G. vaginalis

Belgian isolate 18

-

++++

Atopobium vaginae

CCUG 38953T

-

-

A. vaginae

CCUG 42099T

-

-

A. vaginae

CCUG 44116T

-

-

A. vaginae

Clinical isolate

-

-

Bacillus cereus

-

-

-

Enterobacter aerogenes

CECT 684T

-

-

Escherichia coli O157:H7

NCTC 12900T

-

-

Staphylococcus aureus

CECT 976T

-

-

S. aureus

CECT 86T

-

-

Shigella flexneri

ATCC 12022T

-

-

Listeria monocytogenes

-

-

-

L. monocytogenes

CECT 5873T

-

-

L. seeligeri

CECT 917T

-

-

Klebsiella pneumoniae subsp. ozaenae

ATCC 11296T

-

-

Salmonella Typhi

-

-

-

S. enterica

-

-

-

Escherichia coli

CECT 434T

-

-

Prevotella bivia

ATCC 29303T

-

-

Mobiluncus mulieris

ATCC 26-9T

-

-

Fusobacteria nucleatum

Clinical isolate

-

-

  1. The PNA Probe (Lac663 and Gar162) efficiencies were tested in triplicate experiments for each strain, with the following hybridization PNA FISH qualitative evaluation: (−) Absence of hybridization; (++) Moderate hybridization; (+++) Good hybridization; (++++) Optimal hybridization. The table shows the median value from the three experiments for each strain.