Source tracking of human leptospirosis: serotyping and genotyping of Leptospira isolated from rodents in the epidemic area of Guizhou province, China

Table 1 Information of loci proposed for MLST of leptospiral isolates

Gene	Size of PCR product (bp)	Primer 5’-3’	Annealing temperature (°C)
pntA	638	F: TGCCGATCCTACAACATTA	52
pntA	638	R: AAGAAGCAAGATCCACAACTAC	52
sucA	560	F: AGAAGAGGCCGGTTATCATCAG	52
sucA	560	R: CTTCCGGGTCGTCTCCATTTA	52
pfkB	560	F: CCGAAGATAAGGGGCATACC	52
pfkB	560	R: CAAGCTAAAACCGTGAGTGATT	52
tpiA	534	F: AAGCCGTTTTCCTAGCACATTC	52
tpiA	534	R: AGGCGCCTACAAAAAGACCAGA	52
mreA	602	F: AAAGCGGCCAACCTAACACC	52
mreA	602	R: CGATCCCAGACGCAAGTAAG	52
glmU	557	F: GGAAGGGCACCCGTATGAA	50
glmU	557	R: TCCCTGAGCGTTTTGATTT	50
fadD	577	F: AGTATGGCGTATCTTCCTCCTT	50
fadD	577	R: TTCCCACTGTAATTTCTCCTAA	50

ISSN: 1471-2180