Figure 5

Detection of MMP-2 in supernatant (a) and cellular fraction (c) of HGFs by gelatin zymography and molecular weight positions of pro-MMP-2 (72 kDa) and active-MMP-2 (68 kDa). 5a: Lane1: molecular weight marker; Lane 2: untreated conditioned medium at 48 h; Lane 3: untreated conditioned medium at 24 h; Lanes 4–5: P. gingivalis (Pg) LPS_1435/1449 -treated culture medium at 24 h and 48 h; Lanes 6–7: P. gingivalis LPS₁₆₉₀ -treated medium at 24 h and 48 h; Lanes 8–9: E. coli LPS-treated culture medium at 24 h and 48 h, respectively. 5c: Lane1: Marker; Lanes 2–3: untreated cellular component at 48 h and 24 h; Lanes 4–5: P. gingivalis (Pg) LPS_1435/1449 -treated cellular component at 48 h and 24 h; Lanes 6–7: P.gingivalis LPS₁₆₉₀- treated cellular component at 48 h and 24 h; Lanes 8–9: E-coli LPS-treated cellular component at 48 h and 24 h, respectively. Quantification of band intensities was performed by densitometry analysis using ImageJ software. The fold increase values of MMP-2 in culture supernatant (b) and cellular fraction (d) as compared with the control are shown. *Significant difference (p < 0.05) as compared with the data at 24 h.