From: Role of N-terminal protein formylation in central metabolic processes in Staphylococcus aureus
Gene IDa,b | Nameb | Gene productb | x-fold change |
---|---|---|---|
A | |||
Reduced expression in Δ fmt compared to wild type: | |||
Amino acid metabolism | |||
01452 | ald | alanine dehydrogenase | 103.1 |
00008 | hutH | histidine ammonia-lyase | 67.1 |
01451 | ilvA | threonine dehydratase | 39.8 |
00899 | argG | argininosuccinate synthase | 22.5 |
00435 | gltB | glutamate synthase, large subunit, putative | 21.8 |
02468 | alsS | acetolactate synthase | 14.1 |
00558 | acetyl-CoA acetyltransferase, putative | 12.2 | |
01497 | ansA | L-asparaginase, putative | 7.6 |
01450 | amino acid permease* | 6.4 | |
00081 | HPCH-HPAI aldolase family protein* | 4.6 | |
02287 | leuC | 3-isopropylmalate dehydratase, large subunit | 4.4 |
02574 | NAD-NADP octopine-nopaline dehydrogenase family protein* | 3.8 | |
01450 | amino acid permease* | 3.2 | |
02281 | ilvD | dihydroxy-acid dehydratase | 3.2 |
02839 | L-serine dehydratase, iron-sulfur-dependent, alpha subunit | 2.9 | |
00510 | cysE | serine acetyltransferase, putative | 2.8 |
00147 | acetylglutamate kinase, putative | 2.5 | |
02563 | ureF | urease accessory protein, putative | 2.3 |
02723 | glycerate kinase, putative | 2.2 | |
Protein biosynthesis | |||
01183 | fmt | methionyl tRNA formyltransferase | 585.8 |
01182 | def2* | polypeptide deformylase (def2*) | 6.3 |
01839 | tyrS | tyrosyl-tRNA synthetase | 2.8 |
00324 | ribosomal-protein-serine acetyltransferase, putative | 2.4 | |
01738 | hisS | histidyl-tRNA synthetase | 2.4 |
Folic acid metabolism | |||
01183 | fmt | methionyl tRNA formyltransferase | 585.8 |
02374 | aminobenzoyl-glutamate utilization protein B, putative | 4.5 | |
02610 | hutG | formiminoglutamase | 3.4 |
Fermentation | |||
00188 | pflA | formate acetyltransferase activating enzyme | 604.5 |
02830 | ddh | D-lactate dehydrogenase, putative | 263.6 |
00187 | pflB | formate acetyltransferase (pyruvate-formate-lyase) | 99.0 |
00608 | adh1 | alcohol dehydrogenase I, putative | 74.0 |
00113 | adhE | alcohol dehydrogenase, iron-containing | 40.8 |
02467 | budA2 | alpha-acetolactate decarboxylase | 2.6 |
02875 | L-lactate dehydrogenase, putative | 2.3 | |
Purine metabolism | |||
02553 | inosine-uridine preferring nucleoside hydrolase* | 3.3 | |
00211 | inosine-uridine preferring nucleoside hydrolase* | 3.3 | |
Lipid biosynthesis | |||
01278 | glpD | aerobic glycerol-3-phosphate dehydrogenase | 14.7 |
Transport systems | |||
00748 | iron compound ABC transporter, ATP-binding protein, putative* | 15.0 | |
03019 | ABC transporter, ATP-binding protein, putative | 7.2 | |
01991 | ABC transporter, permease protein, putative | 7.1 | |
00155 | PTS system, glucose-specific component | 5.0 | |
00424 | ABC transporter, permease protein, putative | 3.9 | |
02154 | ABC transporter, ATP-binding protein, putative | 2.6 | |
00844 | ABC transporter, substrate-binding protein* | 2.2 | |
00215 | PTS system component, putative | 2.1 | |
Urea metabolism | |||
00899 | argG | argininosuccinate synthase | 22.5 |
02563 | ureF | urease accessory protein, putative | 2.3 |
energy production and conversion/electrone transfer | |||
00412 | ndhF | NADH dehydrogenase subunit 5, putative | 359.0 |
00302 | NADH-dependent flavin oxidoreductase, Oye family* | 5.2 | |
Higher expression in Δ fmt compared to wild type: | |||
Amino acid metabolism | |||
02971 | aur | aureolysin, putative | 3.4 |
B | |||
Gene ID a,b | Name b | Gene product b | x-fold change |
Reduced expression in Δ fmt compared to wild type: | |||
Amino acid metabolism | |||
00836 | gcvH | glycine cleavage system H protein | 2.4 |
00151 | branched-chain amino acid transport system II carrier protein | 2.4 | |
01452 | ald | alanine dehydrogenase | 2.3 |
01450 | amino acid permease* | 2.1 | |
00510 | cysE | serine acetyltransferase, putative | 2.1 |
01451 | ilvA | threonine dehydratase | 2.1 |
Protein biosynthesis | |||
01183 | fmt | methionyl-tRNA formyltransferase | 158.3 |
01182 | def2* | polypeptide deformylase (def2*) | 4 |
01788 | thrS | threonyl-tRNA synthetase | 3.7 |
00009 | serS | seryl-tRNA synthetase | 2.4 |
01839 | tyrS | tyrosyl-tRNA synthetase | 2.3 |
01159 | ilsS | isoleucyl-tRNA synthetase | 2.1 |
Folic acid metabolism | |||
01183 | fmt | methionyl-tRNA formyltransferase | 158.3 |
00836 | gcvH | glycine cleavage system H protein | 2.4 |
Lipid biosynthesis | |||
01310 | cardiolipin synthetase, putative | 2.8 | |
Fermentation | |||
02830 | ddh | D-lactate dehydrogenase, putative | 9.8 |
00206 | L-lactate dehydrogenase | 2.3 | |
00113 | adhE | alcohol dehydrogenase, iron-containing | 2 |
Increased expression in Δ fmt compared to wild type: | |||
Amino acid metabolism | |||
02840 | L-serine dehydratase, iron-sulfur-dependent, beta subunit | 4.3 | |
Protein biosynthesis | |||
01725 | tRNA methyl transferase, putative | 2.1 | |
Purine metabolism | |||
01012 | purQ | phosphoribosylformylglycinamidine synthase I | 4.2 |
01014 | purF | amidophosphoribosyltransferase | 3.6 |
00372 | xprT | xanthine phosphoribosyltransferase | 3.2 |
Purine metabolism (continued) | |||
00375 | guaA | GMP synthase, putative | 2 |
Lipid biosynthesis | |||
01260 | pgsA | CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase | 2.1 |
03006 | lipase | 2.7 | |
Carbohydrate metabolism | |||
01794 | gap | glyceraldehyde-3-phosphate dehydrogenase, type I | 6.3 |
00239 | ribokinase, putative | 2.1 | |
Riboflavin metabolism | |||
01886 | riboflavin synthase, beta subunit | 25 | |
01888 | riboflavin synthase, alpha subunit | 5.7 | |
01889 | ribD | riboflavin biosynthesis protein RibD | 4.5 |