OmpA-mCherry does not exhibit long-range lateral diffusion. (A) Grayscale image. Note that the poles are brighter than the cylindrical part of the cell. (B) False color images. All images have the same color table (ImageJ Rainbow RGB) and are not contrast enhanced relative to each other. (C) Pixel intensities after background subtraction for both the FRAP ROI (gray symbols) and a non-bleached reference ROI (red symbols) along the filament. Acquisition rate was 2 fps.