OmpA-mCherry is associated with the PG/OM layer. Cells expressing full-length OmpA-mCherry are plasmolyzed in hypertonic sucrose solution. Strain is LMC500. The fluorescence (right) remains associated with the PG/OM layer. Growth conditions: overnight TY culture, diluted 100x in fresh TY grown to exponential phase in 37°C. Plasmolysis is at RT. The phase-contrast image (left) is also depicted inverted-negative (middle) to more clearly visualize the plasmolysis bays.