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Figure 6 | BMC Microbiology

Figure 6

From: Enrichment and characterization of a bacterial culture that can degrade 4-aminopyridine

Figure 6

DGGE profiles of the enrichment culture grown in medium containing 4-aminopyridine, 3,4-dihydroxypyridine, or formate. The enrichment culture grown in medium containing 4-aminopyridine was used to inoculate medium containing 0.9 mM 3,4-dihydroxypyridine or 2.13 mM formate and 0.43 mM ammonium chloride. The culture was incubated and subcultured in fresh medium twice before DGGE analysis. (A) The standard amplified fragments from strains 4AP-A, 4AP-B, 4AP-C, 4AP-D, 4AP-E, 4AP-F, and 4AP-G were loaded in lane M. Lane 1, culture grown in medium containing 4-aminopyridine; lane 2, culture grown in medium containing 3,4-dihydroxypyridine. (B) The standard amplified fragments from the seven strains; lane 1, culture grown in medium containing formate and ammonium chloride; lane 2, culture grown in medium in the absence of formate. Extraction of genomic DNA and preparation of DGGE samples were carried out in triplicate. Prominent DNA bands from the DGGE gels were extracted and used as PCR templates as described in the text.

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