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Figure 3 | BMC Microbiology

Figure 3

From: Characterization of Halomonassp. ZM3 isolated from the Zelazny Most post-flotation waste reservoir, with a special focus on its mobile DNA

Figure 3

Genetic organization of the insertion sequences IS Hsp1 and IS Hsp2 . Inverted repeats (IRL – left IR; IRR – right IR) flanking ISs are marked by black arrowheads. Predicted coding regions are represented by gray arrows indicating the direction of transcription. The location of the DNA-binding domain (HTH) and the DDE motifs are marked. Alignments of the inverted terminal repeats and the sequences of the duplicated direct repeats (DR) are presented beneath each insertion sequence diagram. Identical nucleotides within the IRL and IRR of each IS are indicated by white text against a black background. The amino acid sequences of the predicted N2, N3, and C1 regions, and DDE motifs of the putative transposases encoded by ISHsp1 and ISHsp2 are compared with appropriate family- and group-specific consensus sequences. In the consensus sequences, uppercase letters indicate conservation within the family or group, while lowercase letters denote predominant amino acids, and dashes mark the non-conserved residues. Residues forming the DDE motif are indicated by white text on a black background. The residues conserved in the domains of the analyzed transposases and the consensus sequences are presented against a gray background. The numbers in parentheses show the distances (in amino acids) between the conserved domains.

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