Figure 3

2D gel analysis of M. genitalium total and phosphorylated proteins. Total protein from M. genitalium strains (G37 wild type and TIM207 mutant) were separated in 2D gels and stained with Pro-Q Diamond and Sypro Ruby for the detection of phosphoproteins (gels A and C) and total proteins (Gels B and D), respectively. Protein spots circled and numbered are the ones subjected to mass spectrometry analysis. Protein spots shown in large circles denote the putative high molecular weight proteins showing differential phosphorylation. The sizes (kDa) of protein markers are shown on the right and direction of the first runs are shown by arrows.