Figure 1

Production of recombinant His ₁₀ MG207 protein and determination of phosphatase activity. A. Protein profiles of overexpressed and purified protein of His₁₀MG207. Lanes: Marker, EZ-Run Rec Protein Ladder (Fisher Scientific); Un-induced, extracts of E. coli strain BL21 before the addition of 0.5 mM IPTG; Induced, extracts of E. coli strain BL21 after 3 h of the addition of 0.5 mM IPTG; His₁₀MG207, purified His tagged MG207 protein after Ni-NTA affinity chromatography. Numbers on the left represent the sizes of the marker proteins in KDa. B. Phosphatase activity of His₁₀MG207 with pNPP as substrate. Various amounts (μg) of purified His₁₀MG207 protein (Protein) were added to the reaction mixture containing pNPP. Activity was measured in the presence of 5 mM MgCl₂. Values represent Mean ± SD. C. Phosphatase activity of His₁₀MG207 with synthetic threonine peptide as substrate. Various amounts (μg) of purified His₁₀MG_207 protein (Protein) were added to the reaction mixture containing synthetic threonine phosphate (KRpTIRR). Values represent Mean ± SD.