Figure 6

L. jensenii consistently colonize epithelial model over a 72 h time period in the absence of IL-8 upregulation. Vaginal epithelial colonization of L. jensenii 1153–1666, 2666, 3666, 1646 and gfp bioengineered strains compared with L. jensenii 1153 wild type (WT) strain at the end of 24 h, 48 h, and 72 h, time points. (Figure 6a) Colony forming units (CFU) enumerated from lysates harvested at the end of each 24 h incubation time period. (Figure 6b) Consistent IL-8 profile maintained over time measured in the corresponding supernatants collected at the end of each 24 h incubation. Bars represent mean and SEM from duplicate cultures in four independent experiments. ***P<0.001, **P<0.001 different from medium control, ⁺⁺⁺P<0.001,⁺P<0.001 different from L. jensenii WT.