Figure 4From: Role of the fission yeast cell integrity MAPK pathway in response to glucose limitationPmk1 activation in response to glucose deprivation is independent on the SAPK pathway and requires de novo protein synthesis. A. Strains MI200 (Pmk1-Ha6H; Control), and MI204 (sty1Δ, Pmk1-Ha6H), were grown in YES medium plus 7% glucose to early-log phase and transferred to the same medium with 3% glycerol. Aliquots were harvested at timed intervals and Pmk1 was purified by affinity chromatography. Either activated or total Pmk1 were detected by immunoblotting with anti-phospho-p44/42 or anti-HA antibodies, respectively. B. Control strain MI200 (Pmk1-Ha6H) was grown in YES medium plus 7% glucose to early-log phase, treated with of 100 μg/ml cycloheximide (CHX) for 60 min, and either transferred to the same medium with 3% glycerol (upper panel) or treated with 0.6 M KCl. Purification and detection of active or total Pmk1 was performed as described above.Back to article page