Figure 7
From: Release of extracellular ATP by bacteria during growth
ATP levels in the cultures of Acinetobacter junii . Overnight cultures of two clinical isolates of Acinetobacter junii AJ4970 and AJ4978 were diluted 1:100 in fresh LB broth and cultured at 37°C with shaking. Aliquots were collected at various time points and ATP levels in the culture supernatant and the bacterial pellet were determined. (A) ATP levels in the culture supernatant. ATP concentrations were determined and plotted against the incubation period. (B) ATP levels in the bacterial pellet. Total ATP levels in the bacterial pellet were normalized against OD600nm of each culture and plotted against the incubation time period. (C) Ratio of quantity of ATP in the culture supernatant to that of the bacterial cells. Acinetobacter junii cultures were spun down and separated into culture supernatant and cell pellet. ATP levels in each fraction were determined. The ratio of ATP from supernatant to that of bacterial cells from the same volumes of cultures was plotted against the incubation period. Results are the average of 4 experiments and error bars represent standard deviations.