|
Microbial strains
|
TmP (°C)
|
TmA (°C)
|
|---|
|
Gram positive (G+)
|
Mean
|
SD
|
Mean
|
SD
|
|
Enterococcus faecalis
|
67.94
|
0.07
|
84.14
|
0.36
|
|
Enterococcus faecium
|
67.84
|
0.21
|
84.59
|
0.78
|
|
Listeria monocytogenes
|
67.80
|
0.19
|
86.01
|
0.36
|
|
Staphyloccus aureus
|
64.85
|
0.21
|
83.91
|
0.54
|
|
Staphyloccus epidermidis
|
64.50
|
0.30
|
83.60
|
0.36
|
|
Streptococcus pyogenes
|
46.54
|
0.56
|
84.38
|
0.78
|
|
Gram negative (G-)
| | | | |
|
Acinetobacter baumannii
|
66.09
|
0.15
|
82.90
|
0.16
|
|
Bacteroides fragilis
|
48.65
|
0.18
|
84.47
|
0.84
|
|
Enterobacter aerogenes
|
63.95
|
0.34
|
83.47
|
0.48
|
|
Enterobacter cloacae
|
64.98
|
0.09
|
84.38
|
0.24
|
|
Escherichia coli
|
64.69
|
0.44
|
84.74
|
0.54
|
|
Haemophilus influenzae
|
61.99
|
0.35
|
84.28
|
0.30
|
|
Klebsiella pneumoniae
|
65.13
|
0.23
|
84.57
|
0.20
|
|
Proteus vulgaris
|
64.58
|
0.18
|
82.87
|
0.24
|
|
Pseudomonas aeruginosa
|
53.32
|
0.33
|
83.00
|
0.34
|
|
Serratia marcescens
|
64.01
|
0.30
|
84.17
|
0.30
|
|
Stenotrophomonas maltophilia
|
58.10
|
0.07
|
84.42
|
0.15
|
|
Fungi
| | | | |
|
Candida albicans
|
-
|
-
|
87.1
|
0.33
|
|
Candida dubliniensis
|
-
|
-
|
85.5
|
0.50
|
|
Candida quillermondii
|
-
|
-
|
85.1
|
0.70
|
|
Candida krusei
|
-
|
-
|
89.8
|
0.02
|
|
Candida parapsilosis
|
-
|
-
|
85.4
|
0.88
|
|
Candida tropicalis
|
-
|
-
|
84.5
|
0.75
|
|
Aspergillus fumigatus
|
-
|
-
|
91.0
|
0.38
|
- All the amplicons Tm were measured at the F1 channel (540 nm). The signal was generated by aspecific SybrGreen dye. The G + specific probes produced a signal at the F2 channel (640 nm) the G- probes at the F3 channel (705 nm). The signals were induced with the help of a special FRET technique.
