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Table 1 Melting points of bacterial and fungal amplicons and probes

From: A novel, multiplex, real-time PCR–based approach for the detection of the commonly occurring pathogenic fungi and bacteria

Microbial strains TmP (°C) TmA (°C)
Gram positive (G+) Mean SD Mean SD
Enterococcus faecalis 67.94 0.07 84.14 0.36
Enterococcus faecium 67.84 0.21 84.59 0.78
Listeria monocytogenes 67.80 0.19 86.01 0.36
Staphyloccus aureus 64.85 0.21 83.91 0.54
Staphyloccus epidermidis 64.50 0.30 83.60 0.36
Streptococcus pyogenes 46.54 0.56 84.38 0.78
Gram negative (G-)     
Acinetobacter baumannii 66.09 0.15 82.90 0.16
Bacteroides fragilis 48.65 0.18 84.47 0.84
Enterobacter aerogenes 63.95 0.34 83.47 0.48
Enterobacter cloacae 64.98 0.09 84.38 0.24
Escherichia coli 64.69 0.44 84.74 0.54
Haemophilus influenzae 61.99 0.35 84.28 0.30
Klebsiella pneumoniae 65.13 0.23 84.57 0.20
Proteus vulgaris 64.58 0.18 82.87 0.24
Pseudomonas aeruginosa 53.32 0.33 83.00 0.34
Serratia marcescens 64.01 0.30 84.17 0.30
Stenotrophomonas maltophilia 58.10 0.07 84.42 0.15
Fungi     
Candida albicans - - 87.1 0.33
Candida dubliniensis - - 85.5 0.50
Candida quillermondii - - 85.1 0.70
Candida krusei - - 89.8 0.02
Candida parapsilosis - - 85.4 0.88
Candida tropicalis - - 84.5 0.75
Aspergillus fumigatus - - 91.0 0.38
  1. All the amplicons Tm were measured at the F1 channel (540 nm). The signal was generated by aspecific SybrGreen dye. The G + specific probes produced a signal at the F2 channel (640 nm) the G- probes at the F3 channel (705 nm). The signals were induced with the help of a special FRET technique.