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Table 1 Melting points of bacterial and fungal amplicons and probes

From: A novel, multiplex, real-time PCR–based approach for the detection of the commonly occurring pathogenic fungi and bacteria

Microbial strains

TmP (°C)

TmA (°C)

Gram positive (G+)

Mean

SD

Mean

SD

Enterococcus faecalis

67.94

0.07

84.14

0.36

Enterococcus faecium

67.84

0.21

84.59

0.78

Listeria monocytogenes

67.80

0.19

86.01

0.36

Staphyloccus aureus

64.85

0.21

83.91

0.54

Staphyloccus epidermidis

64.50

0.30

83.60

0.36

Streptococcus pyogenes

46.54

0.56

84.38

0.78

Gram negative (G-)

    

Acinetobacter baumannii

66.09

0.15

82.90

0.16

Bacteroides fragilis

48.65

0.18

84.47

0.84

Enterobacter aerogenes

63.95

0.34

83.47

0.48

Enterobacter cloacae

64.98

0.09

84.38

0.24

Escherichia coli

64.69

0.44

84.74

0.54

Haemophilus influenzae

61.99

0.35

84.28

0.30

Klebsiella pneumoniae

65.13

0.23

84.57

0.20

Proteus vulgaris

64.58

0.18

82.87

0.24

Pseudomonas aeruginosa

53.32

0.33

83.00

0.34

Serratia marcescens

64.01

0.30

84.17

0.30

Stenotrophomonas maltophilia

58.10

0.07

84.42

0.15

Fungi

    

Candida albicans

-

-

87.1

0.33

Candida dubliniensis

-

-

85.5

0.50

Candida quillermondii

-

-

85.1

0.70

Candida krusei

-

-

89.8

0.02

Candida parapsilosis

-

-

85.4

0.88

Candida tropicalis

-

-

84.5

0.75

Aspergillus fumigatus

-

-

91.0

0.38

  1. All the amplicons Tm were measured at the F1 channel (540 nm). The signal was generated by aspecific SybrGreen dye. The G + specific probes produced a signal at the F2 channel (640 nm) the G- probes at the F3 channel (705 nm). The signals were induced with the help of a special FRET technique.