Table 2 Description of primers used in PCR for the detection of virulence markers and erythromycin/clindamycin-resistance genes
Target genea | Sequence of the primer (5′ → 3′) | Amplicon size (bp) | Accession numberb |
|---|---|---|---|
hylB | F: TGTCTCCGAGGTGACACTTGAACT | 124 | U15050.1/Y15903.1 |
R: TTGTGTTGTGACGGGTTGTGGATG | |||
cylE | F: TCGGAACAAGTAAAGAGGGTTCGG | 130 | AF093787.2/AF157015.2 |
R: GGGTTTCCACAGTTGCTTGAATGT | |||
PI-1 | F: AACCACTAGCAGGCGTTGTCTTTG | 147 | EU929540.1/EU929469.1 |
R: TGAGCCCGGAAATTCTGATATGCC | |||
PI-2a | F: GCCGTTAGATGTTGTCTTCGTACT | 117 | EU929374.1/EU929330.1 |
R: TTTACTGCGGTCCCAAGAGCTTC | |||
PI-2b | F: AAGTCTTGACCAAGGATACGACGC | 152 | EU929426.1/EU929391.1 |
R: ATCGTGTTACTTGCCCTGCGTA | |||
ermA | F: CCGGCAAGGAGAAGGTTATAATGA | 190 | EU492925.1/EU492926.1 |
R: GCATTCACCCGTTGACTCATTTCC | |||
ermB | F: GCTCTTGCACACTCAAGTCTCGAT | 117 | EF422365.1/DQ250996.1 |
R: ACATCTGTGGTATGGCGGGTAAGT | |||
mefA/E | F: GCGATGGTCTTGTCTATGGCTTCA | 225 | DQ445273.1/DQ445269.1 |
R: AGCTGTTCCAATGCTACGGAT |