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Table 5 Kinetic parameters for the activities of C-His-Rv2135c with different substrates at pH 5.8

From: Functional characterization of two members of histidine phosphatase superfamily in Mycobacterium tuberculosis

 

Specific activity (mol/min/mg)

Km (mM)

p-Nitrophenol Phosphate

0.23 ± 0.07

10.60 ± 0.07

Phosphoenolpyruvate

0.09 ± 0.002

11.25 ± 0.75

Glycerol-1-phosphate

0.05 ± 0.002

14.00 ± 0.00

ADP

0.00

 

3-Phosphoglyceric acid

0.00

 

Glucose-6-phosphate

0.00

 

Fructose-6-phosphate

0.08 ± 0.009

7.75 ± 0.75