Figure 3

Denaturing gradient gel electrophoresis (DGGE) fingerprints of fungal 18S rRNA gene fragments amplified from stem and leaf DNA templates obtained from four genotypes of Lippia sidoides using two sets of primers - EF4/ITS4 [27],[28] and ITS1f/ITS2 [24],[25]. Lanes 1, 2, 3, 4, 1′, 2′, 3′, 4′ – stem samples and 5, 6, 7, 8, 5′, 6′, 7′, 8′ – leaf samples from genotypes LSID003, LSID006, LSID104 and LSID105, respectively. Lanes marked with M correspond to a 1 kb ladder (Promega). The letter F followed by numbers indicates bands that were extracted from the gels for sequence analysis. The right side shows the corresponding dendrogram obtained after cluster analysis with mathematical averages (UPGMA) and Dice similarity coefficients comparing the fungal 18S rRNA gene fragments amplified from stem and leaf DNA templates obtained from four genotypes of L. sidoides. The genotypes are represented by the three first numbers (LSID - 003, 006, 104 and 105), followed by C or F for stem and leaf samples, respectively, and T1 and T2 corresponding to the replicates.