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Table 1 Strains and plasmids used in this study

From: Modulation of cell wall synthesis and susceptibility to vancomycin by the two-component system AirSR in Staphylococcus aureus NCTC8325

Strain or plasmid Relevant genotype Reference or source
Strains   
NCTC8325 Wild-type NARSAa
RN4220 8325-4 r- initial recipient for modification of plasmids which are introduced into S. aureus from E. coli NARSA
ΔairSR 8325 airSR::ermB This study
CairSR 8325 airSR::ermB pLIairSR This study
DH5α Clone host strain, supE44 ΔlacU169 (φ80dlac ZΔM15) hsdR17 recA1 endA1gyrA96 thi-1 relA1 TransGen
BL21 (DE3) Express strain, F-ompT hsdS B (rB- mB-) gal dcm(DE3) TransGen
Plasmids   
pEasy-blunt simple Clone vector, Kanr Apr b TransGen
pET28a(+) Expression vector with a hexahistidine tag, Kanr Novagen
pEairR pET28a(+) with the airR coding sequence, Kanr This study
pEairS pET28a(+) with the airS coding sequence, Kanr This study
pEC1 pUC18 derivative, source of the ermB gene, Apr Bruckner
pBT2 Shuttle vector, temperature sensitive, Apr Cmr Bruckner
pBTairSR pBT2 containing upstream and downstream fragments of airSR and ermB gene, for airSR mutagenesis, Apr Cmr Emr This study
pLI50 Shuttle cloning vector, Apr Cmr Addgene
pLIairSR pLI50 with airSR ORF and its promoter, Apr Cmr This study
  1. aNARSA, Network on Antimicrobial Resistance in Staphylococcus aureus;
  2. bKanr, kanamycin-resistant; Apr, ampicillin-resistant; Cmr, chloramphenicol-resistant; Emr, erythromycin-resistant.