Table 1 Strains and plasmids used in this study

Strains

NCTC8325

Wild-type

NARSA^a

RN4220

8325-4 r- initial recipient for modification of plasmids which are introduced into S. aureus from E. coli

NARSA

ΔairSR

8325 airSR::ermB

This study

CairSR

8325 airSR::ermB pLIairSR

This study

DH5α

Clone host strain, supE44 ΔlacU169 (φ80dlac ZΔM15) hsdR17 recA1 endA1gyrA96 thi-1 relA1

TransGen

BL21 (DE3)

Express strain, F^-ompT hsdS _B (r_B^- m_B^-) gal dcm(DE3)

TransGen

Plasmids

pEasy-blunt simple

Clone vector, Kan^r Ap^{r b}

TransGen

pET28a(+)

Expression vector with a hexahistidine tag, Kan^r

Novagen

pEairR

pET28a(+) with the airR coding sequence, Kan^r

This study

pEairS

pET28a(+) with the airS coding sequence, Kan^r

This study

pEC1

pUC18 derivative, source of the ermB gene, Ap^r

Bruckner

pBT2

Shuttle vector, temperature sensitive, Ap^r Cm^r

Bruckner

pBTairSR

pBT2 containing upstream and downstream fragments of airSR and ermB gene, for airSR mutagenesis, Ap^r Cm^r Em^r

This study

pLI50

Shuttle cloning vector, Ap^r Cm^r

Addgene

pLIairSR

pLI50 with airSR ORF and its promoter, Ap^r Cm^r

This study