Biochemical and molecular characterization of Treponema phagedenis-like spirochetes isolated from a bovine digital dermatitis lesion

Table 2 Comparison of API-ZYM substrate reactivity profiles of Iowa isolates against other DD isolates and known Treponema strains

	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19
Iowa Isolates 1A, 3A, 4A & 5B*	+	+	+	-	-	-	-	-	-	+	+	-	+	-	-	-	+	-	-
T. phagedenis Kazan*	+	+	+	-	+	-	-	-	-	+	+	-	+	-	-	-	+	-	-
T. phagedenis Reiter§	-	-	-	-	-	-	-	-	-	+	-	-	+	+	-	-	+	-	-
T. denticola (ATCC 35405)*	-	+	+	-	-	-	-	+	-	+	+	+	-	-	+	-	-	-	-
T. denticola (ATCC 35405) #	-	+	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-
T. brennaborense (isolate DD5/3)§	+	+	+	-	-	-	-	-	-	+	+	-	+	-	+	-	+	-	-
T. maltophilum (ATCC 51939)§	+	+	+	-	-	-	-	-	-	+	+	+	-	-	+	-	-	-	+
Bovine isolate V1 & others ¶	+	+	+	-	-^**	-	-	-	-	+	+	-	+	+	-	-	+	-	-
Isolates from UK cattle, Group 1 (x5)†	+	+	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-
Isolates from UK cattle, Group 2 (x14)†	+	+	+	-	-	-	-	-	-	+	+	-	+	+	-	-	+	-	+
Isolates from UK cattle, Group 3 (x4)†	-	+	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-
CA Bovine isolates (x7) ‡	+	+	+	-	-	-	-	-	-	+	+	-	+	+	-	-	+	-	-
Bovine isolate 1-9185MED‡	+	+	+	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-

Enzymes: 1, alkaline phosphatase; 2, C₄ esterase; 3, C₈ esterase lipase; 4 C₁₄ lipase; 5 leucine arylamidase; 6 valine arylamidase; 7 cystine arylamidase; 8, trypsin; 9, chymotrypsin; 10, acid phosphatase; 11, naphtholphosphohydrolase; 12, α-galactosidase; 13, β-galactosidase; 14, β-glucuronidase; 15, α-glucosidase; 16, β-glucosidase; 17, N-acetyl-β-glucosaminidase; 18, α-mannosidase; 19, α-fucosidase.
*As determined in this study, **Isolate T 551B only +.
§ Schrank et al. [27], ‡ Walker et al.[11], ¶ Pringle et al. [17], # Wyss et al. [19], † Evans et al. [16].

ISSN: 1471-2180