atpE gene as a new useful specific molecular target to quantify Mycobacteriumin environmental samples

BMC Microbiology

Table 2 Characteristics of Mycobacterium avium , M. fortuitum , M. intracellulare , and M. chelonae DNA amplification using real-time PCR targeting atpE gene (locus Rv1305 in M. tuberculosis genome)

Real-time PCR characteristics	M. avium	M. fortuitum	M. intracellulare	M. gordonae	M. chelonae
Correlation coefficient r² (%)	93.4	97.4	98.1	99.6	99.8
Efficiencies (%)	119	109	119	97	101
QL (ge/reaction)	<100	<100	<100	ND	<100
DL (95%) (ge/reaction)	ND	ND	ND	ND	6

Ct (cycle threshold) set at 0.02. ND stands for not determined, QL for Quantification Limit, and DL for Detection Limit.

ISSN: 1471-2180