Table 1 Primers used in this study
From: Transcriptional analysis of ftsZ within the dcw cluster in Bacillus mycoides
Primer name | Primer sequence | gene | (a) |
|---|---|---|---|
Northern probes | |||
Zfor | AAAGTWATCGGTGTCGGCGGWGGC | ftsZ | +43 |
Zrev | CAGAAATACCTTGAACCCCTTGGCG | ftsZ | +595 |
Ain | GAACAGCAATGAAATATATGTTG | ftsA | +3 |
N2R | ACCGTCTACAATGAACTGTC | ftsA | +411 |
Primer Extension | |||
prex | GCCCAAACCGCACTCGCAC | ftsW | +95 |
Wrev | AATCCATTCTCTGTACCAATG | murG | +125 |
Rip2 | GTTGCTTAGYAGCCAGTTTC | murG | +1030 |
Qrev | TCTTTARCTTTGGTACACGATC | ftsQ | +52 |
Arev | TCATTAACCATTTCACCAATGATG | ftsA | +80 |
N2R | ACCGTCTACAATGAACTGTC | ftsA | +411 |
ZB | CACCGTGTTCAATCATACGG | ftsZ | +103 |
ZD | ACAACCAAACAACGTCGGCG | spoIIGA | +74 |
ZDbis | CCTAACACAAGCCTCCATC | spoIIGA | +158 |
BigD | CCCAAATGCTGTATACACAATAAGTAACGAG | spoIIGA | +273 |
RT-PCR | |||
Zfin | CTTTTATCGTCTACGACGGTTAC | ftsZ | +1158 |
Zin | CATGTTAGAGTTTGATACTACTC | ftsZ | −1 |
Ain | GAACAGCAATGAAATATATGTTG | ftsA | +3 |
Afin | CCCATAAATAACGGAATGCACG | ftsA | +1297 |
Qin | CGTACATGAARAAYAGTAARG | ftsQ | −5 |
Mbin | GAGATTGTCTATGGAACAATTAG | murB | −10 |
MGin | ACAGCTGAAACNCTTATTCGTG | murG | +964 |
Fw | CATCAGCACCGTATCGRATG | ftsW | +601 |
Mini-ftsZ | (b) | ||
Hind5 | GACAAGCTTATATTGGTGTTCGTGAG | ftsA | +1056 |
Eco5 | GGCGAATTCGCTAATTGATCTTGAG | ftsZ | +39 |
Eco3 | CACGAATTCAAAACAACGTGAAGTTAAG | ftsZ | +1035 |
Bam3 | GGCGGATCCAAAAAGGAGCATGAAAGCTC | spacer | +28 |
Amy5 | GCCGCGATTTCCAATGAGG | pJPR1 | +245 |