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Table 3 Description of the pX1 :: CMY transconjugants obtained from the YU39 donor

From: Conjugative transfer of an IncA/C plasmid-borne blaCMY-2gene through genetic re-arrangements with an IncX1 plasmid

Recipient pX1 ::CMY colony pX1 PCR typing CMY regiona Insertion regionb Second round conjugationc
   oriX1 ydgA taxB taxC ddp3 046-047    Original DH5α
HB101 IC2 + + + + + - Large 046-047 10-1 1 to 10-2
  IIC1 + - + + + + Short ND 10-1 10-1 to 10-2
  IIIC10 + + + + + + Short stbE 10-1 10-1 to 10-2
HB101 (pSTV::Km) ID1 + + + + + - Large 046-047 10-1 1 to 10-1
IIID2 + + + + + - Large 046-047 10-2 to 10-4 10-4 to 10-7
  IVD8 + + + + + + Short stbE 10-1 to 10-2 10-1
LT2 IIE2 + + + + + + Short stbE 10-1 to 10-5 10-1
(pSTV::Km) IIIE4 + + + + + + Large ND 10-4 to 10-7 1 to 10-1
  1. aThe long CMY region includes from ISEcp1 to hypothetical protein 0093, and the short region includes from ISEcp1 to sugE (see text for details).
  2. bSection of pX1 where the CMY region was inserted (see text for details). ND, not determined.
  3. cTransconjugants were challenged for a second round of conjugation using as recipients the original recipient strain (original) or DH5α. The frequency was calculated as number of transconjugants per donor; the range in the orders of magnitude obtained is shown.