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Table 1 Bacterial strains and plasmids used in this work

From: Conjugative transfer of an IncA/C plasmid-borne blaCMY-2gene through genetic re-arrangements with an IncX1 plasmid

Strain Plasmids (kb) Feature
Salmonella   
YU39 (ST213) pA/C (150), p100 (100), pX1 (40), pColE1-like (5), p3 (3) Donor
SO1 (ST19) pSTV::Km (94), p80 (80) Recipient
LT2 (ST19) pSTV::Km (94) Recipient
E. coli   
DH5α   Recipient
HB101   Recipient
HB101pSTV pSTV::Km Recipient
DH5α pA/C Wild-type pA/C, donor
DH5α pA/C, pSTV::Km Stability assays
DH5α pX1 Wild-type pX1
Transconjugants   
SO1   
IA4 pA/C Re-arranged pA/C
IA5 pA/C Re-arranged pA/C
IA9 pA/C Re-arranged pA/C
IIA4 pA/C + pX1 pA/C and pX1 co-integrate
HB101   
IC2 pX1::CMY pX1 with the transposed CMY region
IIC1 pX1::CMY pX1 with the transposed CMY region
IIIC9 pA/C + pX1 pA/C and pX1 co-integrate
IIIC10 pX1::CMY pX1 with the transposed CMY region
IVC8 pA/C + pX1 pA/C and pX1 co-integrate
HB101pSTV ::Km   
ID1 pX1::CMY pX1 with the transposed CMY region
IID2 pX1::CMY pX1 with the transposed CMY region
IIID8 pA/C + pX1 pA/C and pX1 co-integrate
IVD2 pA/C + pX1 pA/C and pX1 co-integrate
IVD8 pX1::CMY pX1 with the transposed CMY region
LT2   
IIE2 pX1::CMY pX1 with the transposed CMY region
IIIE4 pX1::CMY pX1 with the transposed CMY region
IIIE9 pA/C + pX1 pA/C and pX1 co-integrate
DH5α   
221-1 pA/C + pX1 pA/C and pX1 co-integrate
221-10 pA/C + pX1 pA/C and pX1 co-integrate
225-1 pA/C + pX1 pA/C and pX1 co-integrate
225-7 pA/C + pX1 pA/C and pX1 co-integrate
pX1 mutants   
DH5α pX1ydgA::Tn5 Tn5 transposon insertion
DH5α pX1taxB::Km taxB site-directed mutant
DH5α pA/C, pX1ydgA::Tn5 Donor
DH5α pA/C,pX1taxB::Km Donor