From: Cloning and expression of an active aspartic proteinase from Mucor circinelloides in Pichia pastoris
Primer name | Sequence (5′-3′) |
---|---|
Primers for DNA fragment PCR to obtain a partial sequence of genomic DNA of the acid proteinase | |
12 ND-F | AACGATATCGAGTACTATGGT |
M.cir-2R | TTAAAGACTTCATAGTTGTTCTT |
Primer for 3′-RACE PCR (Gene-specific primer) | |
GSP-Mucor-1 F | GATGGTCGTGCCTGGTCTATCCAAT |
Primer for 5′-RACE PCR (gene-specific primer) | |
GSP-Mucor-2R | CATTGTCTCTGGCACCGTATTGAGCAGC |
Primers for full-length cDNA and recombinant plasmids | |
APMC-EcoNaeI-F | ATGGAATTCGCCGGCGCTACTACTGATGCCACTGGTACTGTCCCCG |
APMC-F | AGGAATTCTTCTCATTAGTCTCTTCTTG |
APMC-Met-F | ATGGAATTCATGAAATTCTCATTAGTCTCTTCTTGTGTC |
MCAP-3 F | TATCTCGAGaaaagaGCTCCCAGTGGTAGCAAGAA |
XhoI-N-MCAP-F | TATCTCGAGaaaagaATGAAATTCTCATTAGTCTCTTCTTGTG |
APMC-NotI-R | AAAGCGGCCGCGACAGATTTGGCAATTT |
APMC-Stop-R | GTGATTTATAGATAGATAGATGAAATGTACCAAA |
Primers to identify clones containing recombinant plasmids | |
pGAP-F | GTCCCTATTTCAATCAATTGAACAAC |
AOX1pGAP-Rev | CAAATGGCATTCTGACATCCTC |