Table 1 Purification of Gal308
Purification step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Fold purification | Activity yield (%) |
|---|---|---|---|---|---|
Cell lysate | 37.94 | 1122.21 | 29.58 | 1.00 | 100.00% |
Ni-NTA chromatography | 5.16 | 953.88 | 184.86 | 6.25 | 85.00% |
Purification step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Fold purification | Activity yield (%) |
|---|---|---|---|---|---|
Cell lysate | 37.94 | 1122.21 | 29.58 | 1.00 | 100.00% |
Ni-NTA chromatography | 5.16 | 953.88 | 184.86 | 6.25 | 85.00% |