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Figure 2 | BMC Microbiology

Figure 2

From: Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples of Turpan Basin

Figure 2

SDS-PAGE analysis of recombinant Gal308 from supernatant of E. coli BL21 (DE3) cell lysates and purified Gal308 by affinity chromatography. Lanes: M, standard protein molecular mass markers (sizes in kilodaltons are indicated on the left); 1, recombinant Gal308 from supernatant of E. coli BL21 (DE3) cell lysates; 2, recombinant Gal308 purified by His•Bind® Purification Kit. The sizes in kilodaltons of protein marker were listed as follows: porcine heart myosin (200,000 Da), E. coli β-galactosidase (116,000 Da), rabbit muscle phosphorylase B (97,200 Da), bovine serum albumin (66,409 Da), ovalbumin (44,287 Da), carbonic anhydrase (29,000 Da).

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