Salmonella Typhimurium TTSS-2 deficient mig-14 mutant shows attenuation in immunocompromised mice and offers protection against wild-type Salmonella Typhimurium infection

Table 2 Bacterial strains and plasmids used in the study

Strains	Genetic information	Background	References
SB300	Salmonella Typhimurium, Sm^r	Wild type	[41]
M1525	Salmonella Enteritidis 125109 wild type; Sm^r	Wild type	[42]
MT4	S. Typhimurium ΔssaV,Δmig-14; Sm^r	SB300	This study
MT5	S. Typhimurium ΔssaV; Sm^r	SB300	This study
Plasmids	Relevant genotype (S) and/or phenotype (S)	Resistance	References
pM973	bla PssaH gfpmut2 plasmid with oripMB1	Amp^r	[44]
pKD46	Red recombinase expression plasmid; P_araB; oriR101	Amp^r	[43]
pKD4	Template plasmid; FRT-aphT-FRT	Km^r	[43]
pCP20	FLP recombinase expression plasmid	Cm^r, Amp^r	[43]

ISSN: 1471-2180