Table 1 Bacterial strains and plasmids used in this study
Strain or plasmid | Phenotypes and/or characteristics | Reference or source |
|---|---|---|
B. cenocepacia | Â | Â |
WT | J2315 Wild type strain, Genomovars III of B. cepacia complex | ATCC |
d0581 | BDSF-minus mutant derived from J2315 with Bcam0581 being deleted | 35 |
d0581(0581) | Mutant d0581 harboring the expression construct pMLS7-0581 | 35 |
P. aeruginosa | Â | Â |
PA14 | Clinical isolate | Laboratory collection |
PA14(PCZ) | PA14 harboring the T3SS reporter construct mini-CTX-lacZ with the exsCEBA promoter fused to lacZ | This study |
PA14 (PlasR-lacZ) | PA14 harboring the reporter construct pME2PlasR-lacZ | This study |
PA14 (PpqsR-lacZ) | PA14 harboring the reporter construct pME2PpqsR-lacZ | This study |
PA14 (PrhlR-lacZ) | PA14 harboring the reporter construct pME2PrhlR-lacZ | This study |
dpsrA (PCZ) | psrA deletion mutant harboring the T3SS reporter construct mini-CTX-lacZ with the exsCEBA promoter fused to lacZ | This study |
dPA1396 | PA1396 deletion mutant derived from PA14 | This study |
E.coli | Â | Â |
S17-1 | Res- pro mod+ integrated copy of RP4, mob+, used for incorporating | Laboratory collection |
CV026 | The biosensor strain of C4-HSL | Laboratory collection |
Agrobacterium tumefaciens | Â | Â |
CF11 | AHL reporter strain | Lab of Stephen K. Farrand |
Plasmid | Â | Â |
PCZ | mini-CTX-lacZ with the exsCEBA promoter fused to lacZ | 62 |
PlasR-lacZ | pME2-lacZ containing promoter of lasR | This study |
PpqsR-lacZ | pME2-lacZ containing promoter of pqsR | This study |
PrhlR-lacZ | pME2-lacZ containing promoter of rhlR | This study |